摘要
目的:评价两种不同来源的间充质干细胞,即骨髓干细胞(BMSC)和脂肪干细胞(ADSC)的体外成骨能力,为其将来应用于细胞治疗和组织工程研究提供一定的理论依据。方法:分别取同一只兔的髂骨骨髓和腹股沟脂肪作为BMSC和ADSC的来源,分离培养后比较成骨、成脂分化潜能,细胞表面抗原标记物;成骨诱导后检测碱性磷酸酶(ALP)活性,RT-PCR分析其成骨相关基因的表达情况。结果:BMSC和ADSC都具有向成骨、成脂分化的潜能;在成骨诱导分化后,BMSC有较高的ALP活性和较多的钙结节形成。RT-PCR结果显示:BMSC表达较高的BMP-2、OCN和OPN等成骨基因。结论:BMSC和ADSC都具有向成骨、成脂分化的潜能,ADSC有较好的成脂分化能力,BMSC有较好的成骨分化能力;两者都可以作为组织工程的种子细胞。
Objective： This study was designed to evaluate the osteogenic capacity of rabbit mesenchymal stem cells both isolated from bone marrow （BMSCs） and adipose tissue （ADSCs） in vitro. Methods： BMSCS and ADSCS were obtained from iliac bone marrow and inguinal fat tissue respectively of the same animal. BMSCs and ADSCs were cultured in the medium and their third-passage cells were used for experiments. We compared their multi-differentiation potential, and cell surface antigen markers. Alkaline phosphatase （ALP） activity was assayed as well as to assess gene expression by RT-PCR. Results： BMSCs and ADSCs both have multilineage differentiation potentials. BMSCs expressed highest ALP activity and more calcium nodule formation after osteogenic differentiation. RT-PCR results showed that BMSCs also express higher osteogenic genes like BMP-2, OCN and OPN. Conclusion： BMSCs and ADSCs both have muhilineage differentiation potentials; but compared to the ADSCs which expressed more adipogenic differentiation capacity, the BMSCs osteogenic differentiation capacity is more better. Both of them can be used as seed cells for tissue engineering.
出处
《口腔颌面外科杂志》
CAS
2013年第1期8-13,共6页
Journal of Oral and Maxillofacial Surgery
基金
国家自然科学基金资助项目(81070820
31170942)
陕西省13115科技创新工程重大项目(2009ZDK74)
关键词
干细胞
骨髓基质干细胞
脂肪干细胞
成骨分化
组织工程
stem cell
bone marrow stem cell
adipose-deprived stem cell
osteogenic differentiation
tissue engineering
