弓形虫P22编码基因真核表达质粒接种小鼠后的细胞免疫效果

STUDY ON CELLULAR IMMUNE RESPONSE INDUCED BY IMMUNIZATION WITH A DNA PLASMID ENCODING P22 PROTEIN OF TOXOPLASMA GONDII IN MICE

目的 观察分析弓形虫表面抗原 P2 2编码基因真核表达质粒 (p BK/P2 2 )接种小鼠诱导的细胞免疫效果。方法 采用肌肉注射免疫接种 BAL B/c小鼠 ,5周后 ,取鼠脾细胞作 Con A刺激淋转试验 (MTT法 )及 T细胞亚群的测定。结果 MTT试验中 ,p BK/P2 2免疫组、空质粒 p BK- CMV对照组与 NS对照组的 Con A孔的光密度值与不加 Con A孔的光密度值的差值疫苗免疫组略高于二对照组 ,统计学分析无明显差异 (P>0 .0 5 ) ;CD+4 细胞数量变化无显著性差异 (P>0 .0 5 ) ,而 CD+8细胞数量则明显增多 ,p BK/P2 2免疫组、空质粒 p BK- CMV均高于 NS对照组 ,其差异有显著性 (P<0 .0 1) ;p BK/P2 2免疫组与空质粒组间无显著性差异 (P>0 .0 5 )。结论 p BK/P2 2对 Con A刺激的淋巴细胞增殖功能作用不明显 ;T淋巴细胞亚群测定结果表明 ,p BK/P2 2免疫后小鼠 CD+4 淋巴细胞增殖不明显 ,而

In order to study the cellular response induced by immunization with the DNA plasmid(pBK/p22)encoding the P22 protein of Toxoplasma gondii in mice.After 5 weeks of vaccination,the proliferation activity of spleen T lymphcytes was dectect by MTT assay and the number of CD+ 4/CD+ 8 by indirect immunofluorescent assay.The results of MTT assays had no significant difference among pBK/P22 group,pBK-CMV empty plasmid group and NS control group(P>0.05).The number of CD+ 4T cells shows no obvious increase compared with pBK-CMV and NS groups(P>0.05).However,CD+ 8T cell was significant increased (P<0.01) and the value of CD+ 4/CD+ 8 was decreased.