不同血清学方法对调查现场包虫病人血清的检测及评价

THE EXAMINATION AND EVALUATION ON SERA OF 206 CASES WITH HYDATID DISEASE FOUND IN SURVEY SCENE BY DIFFERENT SEROLOGICAL METHODS

本文应用青海囊型包虫抗原 Dot- EL ISA、IHA和新疆囊型包虫抗原 EL ISA、泡型包虫 EM18抗原 EL IB对流行病学调查现场的 2 0 6例包虫病人血清进行了检测。结果表明青海囊型包虫抗原 Dot- EL ISA和 IHA对囊型包虫病人血清的阳性率分别是 90 .37%和 91.98% ;新疆囊型包虫抗原 EL ISA对囊型包虫病人血清的阳性率为 75 .94% ;三种方法对钙化灶型囊型包虫病人血清的阳性率分别是 77.2 7%、81.82 %和 6 5 .91% ,显著低于其它囊型包虫病人血清的阳性率 ;阴性者主要是单纯性肺脏和肝脏囊型包虫病人血清。3种方法 B超、X线诊断的泡型包虫病人的血清阳性率均为 10 0 .0 %。泡型包虫抗原 EM18-EL IB检测结果表明 ,B超 ,X线诊断的泡型包虫病人血清阳性率为 73.6 8% ;囊型包虫病人血清阳性率为 5 .88% ,其中钙化灶型病人血清阳性率为 15 .91% ;阳性血清数与阴性血清数比例约为 1:7(2 5 :181)。

The sera of 206 cases with hydatid disease diagnosed by B-ultrasound and X-ray in survey scene had been examined by Dot-ELISA and IHA with Qinghai cystic hydatid antigen,ELISA With Xingjiang cystic hydatid antigen and Em18-EliB with alveolar hydatid antigen.The results showed that the sero-positive rates were 9037% and 91.98% in these cases with cystic hydatid disease by Dot EliSA and IHA with Qinghai cystic hydatid antigen respectively.The sero-positive rate was 75.94% in same cases by ELISA with Xingjiang cystic hydatid antigen.The sero-positive rates were 77.27% 81.82% and 65.91% in those cases with the whole calcific cystic hydatid disease by above three methods respectively,and the sero-positive rates were lower in whole calcific cystic hydatid than that in other cystic hydatid disease.The sero-negative cases belonged to cystic hydatid disease which located in lungs of livers alone.The results by EM18-ELIB with alveolar hydatid antigen showed that the sero-positive rates were 73.68% and 5.88% in those cases with alveolar hydatid disease and with cystic hydatid disease diagnosed by B-ultrasound and X-ray respectively,and the sero-positive rate was 15.91% in whole calcific cystic hydatid disease.The ratio of the number of positive seras to that of negative seras was 1 to 7 approximately.The value and mean of different serological methods in diagnosis and judge diagnosis for cystic and alveolar hydatid disease had been discussed.