油酸型急性呼吸窘迫综合征血清细胞因子TNF-α、IL-8、IL-10变化及地塞米松干预的实验研究

Experimental study of dexamethasone intervention on variation of serum cytokines TNF-α,IL-8 and IL-10 in rabbits with oleic acid ARDS

目的:观察地塞米松在不同时间干预对兔油酸型急性呼吸窘迫综合征(acute respiratory distress syndrome,ARDS)血清肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素-8(interleukin-8,IL-8)及IL-10的影响,探讨糖皮质激素治疗ARDS的理论基础。方法:将40只大白兔随机分成正常对照组(n=8),ARDS模型组(n=8)和地塞米松治疗组(n=24)。经耳缘静脉注射油酸复制兔ARDS模型,治疗组再分成3组(每组n=8)分别于即刻、1、2 h注射地塞米松治疗,用放射免疫及酶联免疫吸附试验测定血清中TNF-α、IL-8及IL-10水平。结果:ARDS组兔血清TNF-α、IL-8水平高于对照组(P<0.01);而IL-10水平低于对照组(P<0.01)。经地塞米松治疗后,TNF-α、IL-8水平降低,IL-10水平升高。TNF-α、IL-8水平与ARDS组相比有统计学差异(P<0.01),IL-10水平与ARDS组相比有统计学差异(P<0.01)。并且不同时间应用地塞米松治疗后血清TNF-α、IL-8及IL-10水平有相应的不同变化。结论:TNF-α、IL-8及IL-10在兔油酸型ARDS炎症过程中可能起重要作用。地塞米松可通过干预TNF-α、IL-8及IL-10分泌而减轻肺损伤。同时地塞米松干预时间与炎症的发生、发展存在一定关系。

Objective ： To observe the effects and mechanism of dexamethasone intervention on variations of tumor necrosis factor-α （TNF-α） ,interleukin-8（lL-8） and IL-10 in serum of rabbits with acute respiratory distress syndrome（ARDS） induced by oleic acid at different time points. Methods：Totally 40 rabbits were randomly divided into normal control group（n=8）, ARDS group（n=8） and dexamethasone-treated group（n=24）. Animal model of ARDS was established by intravenous injection of oleie acid to rabbits＇ veins through ear margin. Dexamethasone-treated group was subdivided into three groups （n=8） by injecting dexamethasone at different time points（instantly, 1,2 h）. Levels of TNF-α,IL-8 and IL-10 in serum were measured using radioimmunoassay and enzyme linked immunosorbeng assay（ELISA）. Results：Levels of TNF-α and IL-8 in serum were significantly higher in ARDS group than in normal control group （P〈0.01）. Level of IL-10 in serum was significantly lower in ARDS group than in normal control group（P〈0.01）. After dexamethasone treatment,levels of TNF-α and IL-8 in serum were lowered while level of IL-10 in serum was increased. Levels of TNF-α, IL-8 and IL-10 in serum were statistically different between dexamethasone-treated group and ARDS group （P〈0.01）. Differ ent variations of TNF-α,IL-8 and IL-10 levels in serum were observed when dexamethasone was given at different time points. Conclusions ：TNF-α,IL-8 and IL-10 may play an important role in the inflammatory process of oleic acid ARDS in rabbits. Dexamethasone may relieve lung injuries by intervening the secretion process of TNF-α,IL-8 and IL-10. At the same time,the possible relationships may exist between the progression of inflammation and the intervention time of dexamethasone.