白念珠菌耐氟康唑相关基因点突变的检测

Detection of point mutations in genes related to fluconazole resistance in Candida albicans

目的探讨ERGI1、TAC1、MRR1和UPC2点突变与白念珠菌氟康唑耐药之间的关系。方法收集耐氟康唑的r1念珠菌，尿素裂解法提取DNA，PCR扩增ERG11基因全长以及TAC1、MRR1和UPC2基冈的相应片段，送交测序。结果ERG11基因发现8种点突变，即D116E、Y132H、Y132F、K143Q、F229A、E266D、（M-48E和G464S，其中K143Q此前未见文献报道。UPC2基因发现1种点突变G648D。TAC1和MRR1基凼中均未检测到相应点突变。结论ERG11点突变与耐药的关系比较明确，TACI、MRR1和UPC2点突变与耐药的关系有待进一步研究。

Objective To investigate the relationship of point mutations in ERG11, TAC1, MRRI anti UPC2 genes to flt, eonazole resistance in Candida albicans. Methods This study included 11 fluconazole-resistant Cmldid albicans strains. DNA was extracted from these Candida isolates by urea pyrolysis method. PCR was carried out to amplify the full-length EGRI 1 gene, as well as the corresponding fragments of TAC1, MRRI and UPC2 genes followed by DNA sequencing. Results Eight mutations were detected in the ERGI1 gene, including D116E, YI32H, YI32F, KI43Q, T229A, E266D, G448E and G464S. Among them, KI43Q was a novel mutation and had not been reported. A G648D mutation was detected in the UPC2 gene. No mutations were detected in the TACI or MRRI gene. Conclusions There is a definite association between fluconazole resistance and ERGII mutations, while further research is warranted to clarify the relationship between the mutations of TACI, MRRI, UPC2 genes and resistance to flueonazole.