铜绿假单胞菌医院分布及药敏分析

Analysis of Results in the Determination of Resistance Ofpseudomonas Aures to Antibiotics

目的探讨该院分离的铜绿假单胞菌的耐药情况,为临床控制医院感染及合理用药提供可靠依据。方法从临床标本中分离出铜绿假单胞菌1036株并采用英国先德荧光快速微生物分析鉴定/药敏系统进行鉴定并进行药敏实验,按照美国临床实验室标准化研究所2010年版操作和判读结果采用WHNET5.4软件对结果进行分析统计。结果铜绿假单胞菌以重症ICU检出率最高,其次为神经外科,标本分布以痰液为主,该菌主要对磺胺、庆大耐药,耐药率为75.1%和48.8%。对美罗培南耐药率最低,耐药率2.9%。结论铜绿假单胞菌的耐药性比较严重,医院应加强对感染的预防和控制,合理使用抗生素,延缓细菌耐药性的产生。

Objective To analyze antimicrobial resistance of clinical isolates of Pseudomonas aeruginosa strains from our hospital, for offering reliable reference on clinical control of hospital infection and clinical rational use of medicine. Methods A total of 1036 clinical isolates of P.aeruginosa were collected. The British SENSITITRE AUTOMATED MICROBIOLOGY SYSTEM （SAMS） was adopted to identify these samples and detect antibiotics sensitivity. Results were analyzed by WHNETS.4 according to CLSI 2010. Results The most of department distribution was ICU department, and the second rank in the top list was neurosurgery department . These samples were mainly from sputum. Trimethoprim/Sulfamethoxazole showed the highest antibiotic resistance （75.1%）, followed by Gentamicin （48.8%）. Meropenem displayed the lowest antibiotic resistance （2.9%）. Conclusion Antibiotic resistance of P.aeruginosa was so critical that prevention and monitoring Of its infection should be strengthened, and antibiotics should be applied rationally in clinic to delay the outbreak of P.aeruginosa with antibiotic resistance.