摘要
为菊花在凡纳滨对虾健康养殖、疾病预防和饲料添加方面的开发利用提供参考,通过乙醇回流法提取菊花总黄酮,以提取物为效应物研究其对凡纳滨对虾N-乙酰氨基葡萄糖苷酶(NAGase)活力的影响。结果表明:菊花总黄酮提取物对NAGase活力具有明显的抑制作用,其抑制作用属可逆的竞争型抑制,抑制常数KI为0.35mg/mL。
To provide references for healthy breeding and disease prevention of L. vannamei, exploitation and utilization of feed ingredients, the total flavanoids were extracted from Chrysanthemum by ethanol solution dilution methods, and the effects of extraction from Chrysanthemum on the activity of N- acetylglucosaminidase （NAGase） from L. vannamei were investigated. The result showed that the total flavanoids extraction from Chrysanthemum had significant inhibition effect on NAGase activity. It was competitive inhibitors and its inhibitory constant（K1） was 0.35 mg/mL.
出处
《贵州农业科学》
CAS
北大核心
2013年第2期141-143,共3页
Guizhou Agricultural Sciences
基金
福建省科技厅重点项目"N-乙酰氨基葡萄糖苷酶活力调节在对虾疾病诊控中的应用"(2006N0067)
福建省教育厅科技计划项目"黄酮类化合物与N-乙酰氨基葡萄糖苷酶的相互作用机制研究"(JA12275)
关键词
菊花
总黄酮提取物
凡纳滨对虾
N-乙酰氨基葡萄糖苷酶
抑制机理
Chrysanthemum total flavanoids extraction Litopennaeus vannamei
N-acetylglucosaminidase（NAGase）
inhibitory mechanism
