2011年湖南省某些地区流行性腮腺炎病毒基因特性分析

Analysis on genetic characteristics of mumps virus strains circulating in Hunan province in 2011

目的了解湖南地区流行性腮腺炎（流腮）病毒流行株基因特性及变异情况。方法采用Vero／SLAM细胞分离流行性腮腺炎病毒，通过小疏水蛋白基因（SH基因）的序列分析，与其他基因型参考株进行同源性比较，构建亲缘进化树。结果从2011年湖南多地区上送的16份咽拭子标本中分离到腮腺炎病毒4株，通过基因特性分析，4株毒株均属于F基因型。结论2011年湖南省流行性腮腺炎的流行株为F基因型，未发现基因型变异。

Objective To be acquainted with genetic characteristics and variation of mumps virus strains circulating in Hunan province. Methods Mumps virus （MV） strains were isolated using Vero/ SLAM cells. The small hydrophobic protein （SH） genes of MV isolates were sequenced, and the sequences were analysed phylogenetically between the isolated strains and other reference mumps strains. Results 4 mumps virus strains were isolated from 16 specimens collected in 2011 from different regions of Hunan province. The genotype of isolated strains were supposed to be F type. Conclusion Genotype F is the main genotype of circulating strains in Hunan province in 2011 and there is no variation between genotype.