北京地区HBV基因型的分布及其与临床表现关系的初步研究

The preliminary study of HBV genotype distribution and the relationship with clinical manifestation in Beijing area

目的了解北京地区乙肝患者HBV基因型的分布及其与临床表现的关系。方法从1148份血清中筛选出HBsAg阳性血清200份。利用巢式PCR法扩增血清中HBVDNAS基因进行测序并分型；统计分析基因型分布及与临床表现的关系。结果北京HBV有B、C、D三个基因型，其中B基因型17例（12．5％），C基因型116例（85．2％），D基因型3例（0．02％）；在携带者，慢乙肝患者，肝硬化，肝癌中，B型呈逐步下降c型呈逐步上升的趋势。结论c型与严重肝脏疾病的发生有关，感染B型HBV的患者比感染c型HBV的患者的临床预后要好，对抗病毒治疗的应答较好。

Objective To explore the relationship between HBV genotypes distribution in hepatitis B patients and clinical manifestations in Beijing area. Methods 200 HBsAg positive serum were ehoosen from 1148 serum samples, uscing of nested PCR amplification method, HBV DNA S genes were Sequencing and typing; genotype distribution and the relationship with the clinical manifestation were Statistical analysis. Results In Beijing area, HBV has B, C, D three genotypes, including B gene type 17 cases （ 12. 5% ） , C genotype 116 cases （85.2%） , D genotype 3 cases （0.02%） ; in the carriers, patients with chronic hepatitis B, cirrhosis, liver cancer, B type have been gradually falling, C type have been gradually rising trend. Conclusion Serious liver disease relate with C type, B type patients with HBV infection is better than C type patients in clinical prognosis and antivirus treatment response.