一种新的蛛网膜下腔出血动物模型制作方法

A new animal subarachnoid hemorrhage model

目的建立一种能更接近人脑动脉瘤破裂引起蛛网膜下腔出血（SAH）过程的动物模型。方法通过介入法经股动脉路径穿刺兔颈内动脉，造成SAH，然后将60只实验兔按各时间点（对照组、假手术组、术后0h、3h、6h、12h、1d、3d、7d、14d）分成10组，每组6只，行脑血管造影，然后灌注-固定，处死后取基底动脉切片行HE染色观察，通过图像分析系统，测定动脉内径和血管壁厚度。结果血管内穿刺法制作兔的SAH模型呈急性期脑血管痉挛（SAH后12h）和迟发性脑血管痉挛（SAH后7d）双相改变。结论此动物模型能较好地模拟动脉瘤性SAH后血管痉挛改变的病理过程。

Objective To set up a new experimental model of subarachnoid hemorrhage. Methods A rabbit model was established by endovascular puncture in the internal carotid artery （ICA） through the femoral artery. 60 rabbits were randomly divided into 10 groups （control, sham- operation, 0 h, 3 h, 6h, 12 h, 1 d, 3 d, 7 d, and 14 d after SAH）. After cerebral angiography at different time points, all rabbits underwent perfusion -fixation. The basilar artery cuts were stained with HE for morphologic examinations. The artery diameter and vascular wall thickness were determined by image analysis systems analysis. Results The biphasic process of CVS included acute phase spasms （ 12 h after SAH） and delayed phase spasms （7 d after SAH） in the rabbit model made by the endovascular puncture method. Conclusion The model made by the endovascular puncture method can simulate the pathologic process of CVS after SAH.