摘要
目的:探讨整合素α5β1在胃癌组织中的表达特点及其在胃癌发生、发展中的作用.方法:应用免疫组织化学方法及原位杂交法同步检测80例胃癌组织及50例癌旁正常组织中整合素α5β1表达情况,分析其在胃癌组织中表达的特点以及和临床病理指标的关系.结果:(1)胃癌组整合素α5β1蛋白的阳性表达率为60%,高于癌旁正常组(22%),差异有统计学意义(P<0.05),胃癌组整合素α5β1 mRNA的阳性表达率为61.25%,高于癌旁正常组(24%),差别有统计学意义(P<0.05);(2)整合素α5β1在胃癌组织的表达与胃癌患者的年龄、性别、病理类型无关(P>0.05),与癌组织的分化程度、TNM分期、淋巴结转移、浸润深度密切相关(P<0.05).结论:胃癌组织中整合素α5β1高表达与胃癌的生长、浸润和转移密切相关.
AIM: To investigate the expression of integrin α5β1 in gastric carcinoma and to analyze its relationship with the development and prognosis of gastric carcinoma. METHODS: The expression of integrin α5β1 protein and mRNA was analyzed using immunohistochemistry and in situ hybridization (ISH) in 80 cases of gastric carcinoma and 50 cases of normal stomach tissue. The relationship between integrin α5β1 expression and clinicopathological parameters of gastric carcinoma was ana- lyzed. RESULTS: The positive rate of integrin α5β1 protein expression in gastric carcinoma was significantly higher than that in normal stomach tissue (60% vs 22%, P 〈 0.05). The positive rate of integrin α5β1 mRNA expression in gastric carcinoma was also significantly higher than that in normal stomach tissue (61.25% vs 24%, P 〈 0.05). The expression of integrin α5β1 in gastric carcinoma was closely associated with tumor differentiation, TNM stage, lymph node metastasis, and depth of invasion (all P 〈 0.05), but not with age, gender or pathological type (all P 〉 0.05). CONCLUSION: High expression of integrin α5β1 has a close relation with the growth, invasion and metastasis of gastric carcinoma.
出处
《世界华人消化杂志》
CAS
北大核心
2013年第6期478-483,共6页
World Chinese Journal of Digestology
关键词
胃癌
整合素Α5Β1
免疫组织化学
原位杂交
Gastric carcinoma
Integrin α5β1
Immunohistochemistry
In situ hybridization