儿宝颗粒对幼龄厌食大鼠下丘脑中β-内啡肽含量的影响

Study the Role of β-endorphin in Hypothalamic of Juvenile Rats with Anorexia and the Effect of Erbao Granules on them

目的 :研究 β 内啡肽在幼龄厌食大鼠治疗前后下丘脑的含量变化及其与小儿厌食症的发病关系。方法 :模拟小儿厌食症病因建立幼龄厌食大鼠模型 ,并用运脾方药治疗 ,用免疫组化方法观察正常组、模型组、治疗组下丘脑 β 内啡肽的含量变化 ,结合图像分析技术测定其结果。结果 :模型动物 β 内啡肽低于对照组 (P <0 .0 5) ,儿宝颗粒治疗组下丘脑中 β 内啡肽的含量较模型组显著增高 (P <0 .0 1)。结论 :下丘脑 β 内啡肽的含量降低可能是幼龄厌食大鼠进食量降低的原因之一 ,儿宝颗粒有促进幼龄厌食大鼠下丘脑 β 内啡肽分泌的作用 ,从而增加了摄食量。

Objective:To study the role of β-EP( beta-endorphin) in hypothalamic of juvenile rats with anorexia and the effect of Erbao Granules on them. Methods: A model of juvenile rats with anorexia was established by imitating the cause of children anorexia. Immunohistochemistry and quantitation were used in experiment. Result: The neurone cell of β-EP in hypothalamic in the model group were lower than in the control group (P<0.05). The neurone cell of β-EP in hypothalamic in the therapy group significant increased than the model group (P<0.01). Conclusion: : The decreasing of β-EP in hypothalamic may be one of the factors causing anorexia in rats. Erbao granules can improve β-EP in hypothalamicof anorexia.