大鼠肝癌细胞C2不同途径接种造模及比较

Establishment and comparison of liver cancer models produced by different injection routes of rat C2 hepatocellular carcinoma cells

目的探讨大鼠肝癌细胞系C2肝、脾原位接种成瘤及肠系膜静脉注射在不同内环境情况下对肿瘤发生和侵袭转移能力的影响,并筛选最佳C2肝细胞癌肺转移动物模型。方法 C2进行体外培养。将雌性BALB/cA裸鼠随机分为肝、脾原位接种及肠系膜静脉注射3组。观察裸鼠肝、脾成瘤率、裸鼠存活率、肿瘤病理学特点及远处脏器转移率及强度。结果肝原位接种组成瘤率为100%,肿瘤呈结节状膨胀性生长,周围肝组织和腹壁有粘连和侵袭,肺转移率为100%。脾原位接种组脾成瘤率为50%,肉眼未见明显的肿瘤形成,但部分肝表面可见转移灶;镜下肝、肺肿瘤转移率分别为67%和33%。肠系膜静脉注射组肉眼未见转移灶,镜下示肝、肺肿瘤转移率均为25%。结论三种不同方法构建的C2肝癌肺转移动物模型均有肺转移灶的出现,其中,肝原位接种模型最适合于肝细胞癌体内及肺转移机制及药物干预研究。

Purpose To establish liver cancer models in nude mice by injection of rat C2 hepatocellular carcinoma （HCC） cells via different routes, and to study the carcinogenic and metastatic phenotypes in these liver cancer models. Methods The C2 HCC cells were cultured in vitro first, and then were injected into liver, spleen lobe, or the mesenteric vein of female BALB/eA nude mice. Thereafter, the tumor formation rate, carcinogenic features, and metastasis were comparatively studied. Results In the mice with di- rect liver injection of C2, all animals developed liver cancer with 100% lung metastasis. The tumor grew fast, and exhibited an expan- sive growing pattern with surrounding invasion. In the mice with spleen injection of C2, 50% animals developed microscopically hepa- tocellular spleen tumor, with a 67% liver and a 33% lung metastatic rate. In the mice with mesenteric vein injection of C2, only 1 ani- mal developed microscopically liver and lung metastasis. Conclusion All the injection routes tested here produce carcinogenesis or metastasis in the liver, and lung metastasis, while the carcinogenic rate and the lung metastatic rate are different. The direct liver in- jection model is the most modeling method, based on our studies, and therefore, it may serve as a valuable model for the studies of the underlying mechanism of carcinogenesis and metastasis, and also the therapeutic effect on liver cancer.