食管鳞癌XRCC3和HOGG1表达与放疗预后相关性分析

Analysis of relationship between protein expression of XRCC3 and HOGG1 and prognosis in patientswith esophageal squamous cell carcinoma after radiotherapy

目的探讨食管鳞状细胞癌（ESCC）癌组织中x射线修复交叉互补基因3（XRCC，）和人类8-羟基鸟嘌呤糖苷酶1（HOGG，）的蛋白表达与ESCC放疗预后相关性。方法采用免疫组织化学SP法检测治疗前171例ESCC组织标本中XRCC1、HOGG，蛋白表达。Kaplan-Meier法生存分析并Logrank法检验表达阴、阳性间生存差异，Cox模型多因素预后分析。结果随访率为87．2％，其中随访时间满1、2、3年者分别为140、136、129例。XRCC，主要表达于细胞核，HOGG．主要表达于细胞核与线粒体，两者符合程度达72．5％（妒=23．94，P=0．000）。XRCC，表达阳、阴性的近期疗效相似（X2=0．98，P=0．614），生存率也相似，中位生存期均为54个月（X2=0．17，P=0．683），HOGG，表达阳、阴性近期疗效相似（X2=0．26，P=0．880），生存率也相似，中位生存期均为49个月（X2=0．08，P=0．780）。多因素分析结果显示疗效评价、病变长度与ESCC预后相关（妒=7．99、3．76，P=0．005、0．045）。结论XRCC3、HOGG1的蛋白表达与ESCC放疗预后可能无关。

Objective To investigate the relationship between the protein expression of X-ray repair cross-complementing protein 3 （ XRCC3 ） and human 8-hydroxyguanine glycosylase 1 （ HOGG1 ） in esophageal squamous cell carcinoma （ ESCC ） tissue and the prognosis in ESCC patients after radiotherapy . Methods Immunohistochemieal SP method was used to measure the protein expression of XRCC3 and HOGGl in 171 ESCC tissue samples before radiotherapy. The Kaplan-Meier method was used for survival analysis, and the logrank test was used for analyzing the survival difference between negative and positive samples. The Cox model was used for multivariate prognostic analysis. Results The follow-up rate was 87.2% ; 140 patients were followed up for at least 1 year, 136 patients for at least 2 years, and 129 patients for at least 3 years. XRCC3 was mainly expressed in the nucleus, and HOGGj was mainly expressed in the nucleus and mitochondria, with a coincidence degree of 72. 5% （ X2 = 23.94, P = 0. 000 ）. The patients with positive XRCC3 expression and negative XRCC3 expression had similar short-term responses （ X2 = 0. 98, P = 0. 614） as well as similar survival rates, and both patient groups had a median survival time of 54 months （ X2 = 0. 17, P = 0. 683 ）. The patients with positive HOGG1 expression and negative HOGG1 expression had similar short-term responses （ X2 = 0. 26, P = 0. 880） as well as similar survival rates, and both patient groups had a median survival time of 49 months （ X2 = 0. 08, P = 0. 780 ）. The multivariate prognostic analysis showed that the response evaluation and tumor length were related to the prognosis of ESCC （ X2 = 7.99, P = 0. 005 ; X2 = 3.76, P = 0. 045 ）. Conclusions The protein expression of XRCC3 and HOGG1 may be unrelated to the prognosis of ESCC after radiotherapy.