摘要
利用基因枪将无选择标记的优质高分子量麦谷蛋白亚基基因1Dx5导入新疆耐盐小麦品种新冬26,为利用优质基因进行小麦品质改良奠定基础。构建无选择标记的线性1Dx5表达框。利用基因枪将其转入不含该亚基的小麦品种新冬26幼胚盾片中,经PCR二分法筛选,从转化的1 000块幼胚盾片中共获得3株转基因阳性植株,转化效率0.3%。利用SDS-PAGE分析目的基因在转基因后代籽粒中的表达。转基因植株后代种子分析表明,1Dx5在转基因后代部分种子中表达。本研究成功地将无选择标记的线性1Dx5片段导入普通小麦新冬26中,并在后代部分种子中得到了表达。为利用优质亚基基因改良小麦加工品质奠定基础。
To improve the bread-making quality of wheat, the Xinjiang winter common wheat Xindong No. 26 were transformed with high-molecular-weight glutenin subunits (HWM-GS) gene 1Dx5 that without selectable markers by particle bombardment. Transformed plants were screened by PCR, three transgenic plants were detected among 1 000 transformed plants, yielding a transformation efficiency of 0.3%. The compositions of HWM-GS were analyzed by SDS-PAGE. The HMW-GS gene 1Dx5 was expressed in some seeds of transgenic plants. The high molecular weight glutenin subunit gene 1Dx5 was transformed into wheat XinDong No. 26 and was expressed in some transgenic progenies. Our studies lay the foundations for the improvement of bread-making quality of wheat.
出处
《植物研究》
CAS
CSCD
北大核心
2013年第2期191-196,共6页
Bulletin of Botanical Research
基金
国家自然科学基金(30960044)
新疆师范大学研究生创新基金项目资助(项目名称:小麦遗传转化体系建立及高分子量麦谷蛋白亚基基因1Dx5的基因枪转化
项目编号:20121208)
关键词
小麦
选择标记
1Dx5
基因枪
wheat
selectable markers
1 Dx5
microprojectile