何首乌饮对运动疲劳大鼠睾丸组织Cox7a2表达的影响

Effect of Heshouwuyin on the Expression of Cox7a2 Protein in Testis Tissue of Exercised-induced Fatigue Rat

目的探讨中药何首乌饮对运动疲劳大鼠睾丸组织细胞色素C氧化酶7a2(Cox7a2)表达的影响。方法 50只雄性SD大鼠随机分为5组:A组为安静对照组,B组为安静何首乌饮组,C组为运动疲劳模型组,D组为何首乌饮治疗组,E组为何首乌饮预防组,每组10只。C、D和E组大鼠复制运动疲劳动物模型,其中D组于造模期间(42d)每天训练后灌胃何首乌饮20g/(kg.d)(含生药9.6g/mL),造模成功后继续灌胃18d(剂量同前),共60d;E组于造模前18d每天灌胃及造模期间(42d)每天训练前灌胃何首乌饮20g/(kg.d)(含生药9.6g/mL),共60d。采用美国Beckmancoulter Unicel Dxl 800测定大鼠血清睾酮水平,严格按照说明书操作。分别采用RT-PCR、Western blot和免疫组织化学法检测各组大鼠睾丸组织Cox7a2的表达变化。结果 C组大鼠血清睾酮浓度较A组下降(P<0.05),确认模型成功;B、D、E组大鼠血清睾酮浓度则高于A组(P<0.05)。Cox7a2免疫组化阳性颗粒主要表达于间质细胞和精原细胞的胞质、胞核,C组阳性信号较强,A、B组最弱。Cox7a2蛋白和mRNA表达变化为:C组高于其余4组(P<0.05),A、B组之间和D、E组之间差异无统计学意义(P>0.05)。结论何首乌饮可以降低运动疲劳大鼠睾丸组织Cox7a2的表达。

Objective To explore the effect of Heshouwuyin on the expression of cytochrome C oxidaseTa2 （CoxTa2） in testis tissue of rats with exercised induced fatigue. Methods Fifty SD rats were divided into normal control group （A group）, Heshouwuyin administered normal group （B group）, model control group （C group）, Heshouwuyin treated group （D group） and tleshouwuyin prevented group （E group） randomly with 10 rats for each. The exercise-induced fatigue models in rats of C, D, E groups were established. The rats in D group were treated with Heshouwuyin [20 g/（kg · d）, contained crude drug 9.6 g/mL] for 60 days （during the 42 days of modeling and after the 18 days of modeling）. The rats in E group were also treated with Heshouwuyin for 60 days （but before the 18 days of modeling and during the 42 days of modeling）. Beckmancoulter Unicel Dxl 800 was used to detect the level of serum testosterone, according to the manufacture＇s instructions. Western blot and RT-PCR were used to observe the differential expression of CoxTa2. Results The level of serum testosterone in C group was decreased compared with A group （P〈0.05）, which implied the success of modeling. Compared with group A, the level of serum testosterone in B, D, E groups were increased （P〈0.05）. Cox7a2 protein was expressed mainly in leydig cell and spermatocyte. Compared with A,B, D, E groups, the expression of CoxTa2 protein and mRNA in C group increased （P〈0.05）, and there no significant difference was observed between group A and B, as well as group D and E. Conclusion The expression of CoxTa2 was down-regulated by Heshouwuyin.