芬太尼和舒芬太尼对兔离体子宫平滑肌自发性收缩的影响

The Effect of Surfentanyl and Fentanyl at Rhythmic Spontaneous Contraction on Isolated Rabbits Uterine Smooth Muscles

目的观察芬太尼和舒芬太尼对兔离体子宫平滑肌自发性收缩的影响。方法将30条(30只兔)已建立自发性规律收缩的离体兔子宫平滑肌肉条随机分为对照组(C组)、芬太尼组(F组)和舒芬太尼组(S组),每组10条。记录不同浓度的芬太尼和舒芬太尼对兔离体子宫平滑肌自发性收缩强度、频率和时程的影响。结果芬太尼浓度为1×10-8 mol/L时,对兔离体子宫平滑肌的自发性收缩无影响。当芬太尼浓度达到3×10-8 mol/L及以上时子宫平滑肌的收缩强度明显下降(P<0.05),当浓度≥3×10-7 mol/L时,可抑制子宫平滑肌的收缩强度、频率和时程均达50%以上(P<0.05)。舒芬太尼浓度≥1×10-7 mol/L时,可抑制子宫平滑肌收缩时程达50%以上(P<0.05)。与舒芬太尼组比较,当芬太尼浓度≥3×10-8 mol/L时,子宫平滑肌自发性收缩强度差异有统计学意义(P<0.05);当浓度≥3×10-7 mol/L时,子宫平滑肌自发性收缩频率差异有统计学意义(P<0.05);当浓度为1×10-7和1×10-5 mol/L时,子宫平滑肌自发性收缩时程差异有统计学意义(P<0.05)。结论芬太尼呈浓度依赖性抑制兔离体子宫平滑肌的自发性规律收缩。高浓度舒芬太尼可导致离体兔子宫平滑肌的自发性规律收缩的时程缩短,但对子宫平滑肌的自发性规律收缩的强度和频率无影响。

Objective To investigate the effects of surfentanyl and fentanyl on spontaneous contractions of isolated uterine smooth muscles of rabbits. Methods Thirty isolated uterine smooth muscles strips of rabbits with rhythmic spontaneous contraction were randomly divided into three groups： control group （n： 10） fentanyl group （n： 10） and surfentanyl group （n： 10）. The effects of fentanyl and surfentanyl on spontaneous contractions intensity, frequency and duration of isolated uterine smooth muscles of rabbits in different concentrationswere monitored. Results There were no effect on the spontaneous contraction of isolated uterine muscles of rabbits at the concentration of 1 X 10 s mol/L in fentanyl group. The spontaneous contraction intensity was decreased significantly when the concentration of fentanyl more than 3X 10 8 mol/L （P〈0.05）. The spontaneous contraction intensity, frequency and duration was inhibited more than 50 when the concentration of fentanyl more than 3 X 10-7 mol/L （P〈0.05）. And the spontaneous contraction duration was inhibited more than 500.% when the concentration of surfentanyl more than 1 X 10 7 mol/L （P〈0. 05）. Compared with surfentanyl group, there were significant difference on spontaneous contraction intensity when the concentration of fentanyl more than 3 X 10-s mol/L （P〈 0.05）. Difference on frequency, more than 3 X 10 s mol/L （P 〈 0. 05） and difference on duration, at the concentration of 1 ）〈 10 7 and 1 X 10 7 mol/L （P 〈 0. 05）. Conclusion Fentanyl restrained the spontaneous contractions of isolated uterine smooth muscles of rabbits in concentration-dependent manner. Surfentanyl could reduce the spontaneous contractions duration on isolated uterine smooth muscles of rabbits. But there were no effect on frequency and duration.