肺腺癌细胞中EGFR蛋白的表达与细胞内胶原化的相关性研究

Correlation between protein expression of epidermal growth factor receptors and intra-cellular matrix collagenation in lung adenocarcinoma cells

目的探讨表皮生长因子受体(epidermal growth factor receptor,EGFR)在肺腺癌细胞中的表达及与细胞发生胶原化的相关性。方法从胸水中提取肺腺癌细胞为研究对象,以32例良性胸水中的增生上皮细胞、炎性细胞为对照,采用免疫细胞化学方法检测细胞中EGFR、E钙粘素蛋白、Vimentin、TTF-1和胶原蛋白亚型I的表达。Masson染色方法检测胶原纤维表达。结果 78例胸水标本中,EGFR在肺腺癌细胞中的阳性率为79.5%,胶原蛋白亚型I为32.1%,Masson染色的阳性率为70.5%,明显高于对照组且EGFR和Masson染色的阳性表达结果的相关性具有统计学意义(P<0.01)。结论 EG-FR在肺腺癌细胞中阳性表达,可能与细胞内基质胶原蛋白形成有关。

] Objective To investigate the clinical significance of the relationship between EGFR （epidermal growth factor receptor） and intra-cellular matrix collagenation in lung adenocarcinoma cells. Methods Lung adenocarcinoma cells were obtained. 32 cases of hyperplasia epithelial cells of non-malignant pleural effusion were obtained as control. Immunocytoehemiscal staining was used to test EGFR, E- cadherin, Vimentin, TTF-1 and Collagen I. Collagen was analyzed by Masson staining. Results EGFR positive expression was found in 62 of 78 （79. 5%） cases of malignant pleural effusion. Positive rate of Collagen I and Masson staining was 32. 1% and 70.5%. Our data showed that elevated levels of EGFR were accompanied by collagen expression in lung adenocarcinoma cells. Hyperplasia epithelial cells from non-malignant pleural fluid were uniformly negative for EGFR. Conclusion Our study shows EGFR positive expression in lung adenocarcinoma cells may be related to collagen formation in the cytoplasm.