摘要
哺乳动物卵母细胞的体外成熟培养技术与显微实时摄影技术相互结合,使得体外培养卵母细胞成熟过程的全程记录监控成为可能。本研究利用这一技术对牛卵母细胞体外培养过程的形态学变化进行了观测并进行了一些初步分析,用以监测卵母细胞体外成熟的过程,初步探索掌握牛卵母细胞体外成熟过程的形态学变化规律,为相关领域的深入研究提供参考。在CO2培养箱内、外分别进行牛卵母细胞的体外成熟培养,在确定环境对卵成熟影响的基础上,利用显微实时摄像系统监控牛卵母细胞体外培养过程中的形态变化与第一极体排出率。结果表明,在CO2培养箱内、外的不同环境下培养,牛卵母细胞成熟率没有显著差异(P>0.05)。通过实时监测卵母细胞成熟过程,发现部分卵母细胞存在一个短时间的胞质膨涨并随之恢复原体积的过程,而且这类卵母细胞最终死亡,造成这种现象的原因推测是与卵子中一种叫做AQP3的水通道蛋白有关。
Combination of the IVM of mammalian oocytes and microstructure of real-time photographic technique makes the monitoring of the entire process of oocyte IVM possible. We observed morphological changes of bovine oocytes cultured in vitro using this technology and made preliminary analysis. The purpose of this study is monitoring oocyte maturation process in vitro, exploring and mastering the morphological variation pattern of bovine oocytes maturation process in vitro, so as to provide a reference for in-depth study of related fields. Bovine oocytes matured in vitro inside and outside CO2 incubator, respectively. Base on confirming the impact of environment on the oocytes maturation, microscopic real-time camera system was used to monitor morphological changes of bovine oocytes maturation process in vitro and the rate of the first polar body emission in the process. The results showed that bovine oocyte maturation rate indicated no significant differences（ P 〉 0.05 ） under different environments such as inside or outside the CO2 incubator. Through real-time monitoring oocyte maturation process, we found that part of oocyte cytoplasm would swell and restore subsequently in a short period of time, and then died eventually. The cause of this phenomenon was presumably associated with a called AQP3 water channel protein in oocytes.
出处
《生物技术进展》
2013年第1期32-37,共6页
Current Biotechnology
基金
国家自然科学基金项目(31172280)资助
