植物化学诱导基因表达系统研究进展

The Development of Chemical Induced Gene Expression System in Plants

随着植物基因工程的发展,植物的化学诱导表达系统受到越来越多的重视。化学诱导表达系统通常包含两个元件,第一个元件多为一个嵌合的转录因子,可以特异性的结合到启动子上;第二个元件具有前面的转录因子的结合位点,从而控制目的基因的表达。本文综述了当前广泛应用的一元诱导基因表达系统,如四环素诱导表达系统,和二元诱导表达系统,包括:乙醇诱导、地塞米松诱导、β2雌激素诱导和热休克诱导等不同系统,总结了各化学诱导表达系统的最新研究进展及其潜在利用的可行性。

With the development of plant genetic engineering, the application of plant chemical induced gene expression system is becoming more and more important. Chemical induced gene expression system typically consists of two components, the first component is mostly a chimeric transcription factor which can specific bind to the promoter. The second element has a transcription factor binding site for the first element, and thereby controlling the expression of target genes. Here, we review the current widely used one component inducible gene expression system, such as the tetracycline inducible expression system, and two components inducible expression systems, including： ethanol-induced, dexamethasone-indueed, β2 estrogen hormone- induced and heat shock-induced systems. This paper mainly discusses the recent progress on chemical inducing gene expression systems and their potential use in plants.