通过HSQC和~1H,^(13)C-COSY叠合增强二维谱分辨率的方法

A Method for Improving the Resolution of Two-Dimensional NMR Spectrum by Overlapping HSQC and ~1H,^(13)C-COSY

通常二维谱F1维分辨率较差,F2维分辨率较好.即HSQC实验13 C维度分辨率较差,1 H维度分辨率较好.而1 H,13 C-COSY实验13 C维度分辨率较好,1 H维度分辨率较差.提出一种通过HSQC和1 H,13 C-COSY叠合,取较弱的值储存并显示的方法增强分辨率,采用Bruker Topspin软件AU程序实现.该方法在不明显增加采样时间的情况下,可显著增强分辨率,信噪比也没有降低.同时抑制了F1噪声.处理后,谱图美观清晰.

In traditional two-dimensional （2D） NMR experiments, spectral resolution in the F, dimension is usually higher than that in the F2 dimension. Therefore, in the HSQC experiments, the spectral resolution in the ^13C dimension is lower than that in the ^1H dimension. This is the opposite in the ^1H, ^13C-COSY experiments. In this work, a method for improving the resolution of 2D NMR spectrum was presented, in which HSQC and ^1H, ^13C-COSY spectra were overlapped, and then the weaker points were selected, saved and displayed. The method was implemented as an AU program within the Bruker Topspin framework. It was demonstrated experimentally that the method could improve spectral resolution, at no cost of signal-to-noise ratio. The meth-od can also be used to suppress F1 noise.