恶性浆膜腔积液细胞块在分子病理诊断中的应用

Malignant serous cavity effusion cell blocks in the application of molecular pathological diagnosis

目的探讨恶性浆膜腔积液细胞块技术在分子病理诊断上的应用价值。方法 85例恶性浆膜腔积液分层离心获取肿瘤细胞并制作成石蜡细胞块,应用常规病理染色、免疫组化染色、荧光原位杂交(FISH)、基因测序等方法对其进行检测。结果细胞块切片中肿瘤细胞丰富、集中,细胞结构清晰,并保持部分组织学形态特征,免疫组化染色能够辅助肿瘤确诊及明确分型,荧光原位杂交和基因测序可提供基因扩增及突变情况。30例非小细胞肺癌样本中10例EGFR基因的FISH阳性,8例EGFR基因突变。结论恶性浆膜腔积液细胞块技术对于疾病的诊断有重要临床意义,可以帮助肿瘤确诊并查找原发灶,有效指导肿瘤分子靶向治疗。

Objective To evaluate the value of malignant serous cavity effusion cell blocks in the application of molecular pathological diagnosis. Methods Tumor cells were collected and embedded into cell blocks through stratified centrifugal from 85 cancer patients with malignant serous cavity effusion, then were detected by immunohistochemistry, fluorescence in situ hybridisation （FISH） and gene sequencing methods. Results The tumor cells of cell block section were rich and remained histological structures. Immunohistochemistry staining could assist diagnosis and tumor parting. FISH and gene sequencing could provide gene amplification and mutation situation. Ten caces of EGFR gene amplification and 8 caces of EGFR gene mutation were observed in 30 non-small cell lung cancer samples. Conclusion Malignant serous cavity effusion cell blocks are useful for the diagnosis and determing the primary source of tumor, which can instruct targeted therapy.