袖状胃切除术Wistar大鼠模型建立及初步观察

Establishment and preliminary observation on sleeve gastrectomy model in Wistar rats

目的探讨袖带胃切除术对Wistar大鼠体质量、大鼠前胃黏膜及黏膜肌层厚度的影响,为进一步研究袖状胃切除术的减重机制提供形态学依据。方法 20只雄性Wistar大鼠被随机分为2组,12只行袖状胃切除术做为手术组;8只行胃干预组做为假手术组,术前、术后第1天及术后1月内每周,而后每两周进行体重测量,术后12周处死大鼠后制作前胃石蜡切片,组织学方法观察其形态变化。结果手术组大鼠存活10只,成活率83%。假手术组大鼠在术后增重明显,手术组大鼠体重在术后2周恢复术前水平,体重增长明显但较假手术组慢(P<0.05)。手术组大鼠术后前胃有代偿性扩张,光镜下可见黏膜及黏膜肌层厚度变薄,黏膜皱襞变浅。结论袖状胃切除术大鼠模型中对前胃的形态学观察可能有利于进一步阐明该术式大鼠模型的减重和体重反弹机制。

Objective To explore the effect on the body weight and the mucosa and muscularis mucosae thickness of the forestomach on sleeve gastrectomy model in Wistar rat, and to provide morphology base for the further study weight loss mechanisms of sleeve gastrectomy. Methods 20 male Wistar rats were randomly divided into two groups, 12 wistar rats underwent sleeve gastrectomy as the operative group. 8 male Wistar rat underwent a laparotomy and stomach manipulation as the sham operative group. Preoperative weight of the rats was measured, Preoperative, postoperative first day and every week within postoperative 4 weeks, and then every two weeks for weight measurement. Rats were killed 12 weeks after surgery, then made Paraffin sections, observed the morphological changes of forestomach by histological methods. Results All rats remained alive in the sham operative group, 10 rats remained alive in the operative group. In the Sham operative group, weight gain was achieved obviously after operation, whereas in the operative group, weight gain was achieved until the 2th week. In the operative group, weight gain was significantly lower than in the Sham operative group （P 〈 0.05）. Postoperative forestomach in the operative group rats compensatory dilatated, under light microscope mucosa and muscularis mucosa thickness thinning, mucosal shallowing. Conclusion Observation on the forestomach morphology on Sleeve gastrectomy model in Wistar rat may contribute to the further elucidation of weight loss and weight rebound mechanism in the rat model.