隔离器饲育SPF鸡微生物质量控制方法的建立

Establishment of microbiological quality control methods on breeding SPF chickens raised in the isolators

目的建立隔离器饲育SPF鸡生产管理方式,探讨并建立其微生物质量控制方法。方法对引进的SPF鸡种蛋进行孵化后,将种鸡置于隔离器中进行饲养,在种鸡10、20、40周龄时采取鸡血清进行微生物质量监测,检验当前SPF鸡生产管理和微生物质量控制方法的有效性。结果隔离器饲育SPF鸡初期由于对孵化环节控制不严出现了微生物污染现象。在对孵化环节操作管理改进后,隔离器饲育SPF鸡的微生物状况得到控制。结论通过加强各个环节消毒,隔离器饲育环境能够保证SPF鸡的微生物质量。适当控制隔离器内SPF鸡的密度不仅有利于SPF鸡的繁育和种蛋回收,而且有利于环境微生物的控制。

Objective To establish production management and the methods of microbiological quality control on breeding SPF chickens raised in the isolator. Methods The chickens were bred in the isolator after the eggs from introduced SPF chickens were hatched out. The sera obtained from 10, 20, 40-week-old SPF chickens were used to monitor microbiological quality in order to detect the effectiveness ,of the current production management and the methods of microbiological quality control on SPF chickens. Results Because the hatching process was not strickly controlled during the early stage of breeding SPF chickens in the isolator, there were the emergence of microbiological contamination. Microbiological condition of the isolator breeding SPF chickens was effectively controlled after improving the operation management during the hatching process. Conclusion If the disinfection during all breeding stages is strengthened, the microbiological quality of SPF chickens in the isolator could be ensured. The appropriate control on the density of SPF chickens in the isolator is not only beneficial to the breeding of SPF chickens and the recycling of hatching eggs, but also helpful to the control of environmental microbiology.