摘要
Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus （WSSV） envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a con- stitutive secretory expression plasmid as the expression vector. With the ability to deliver recombinant pro- tein products outside, DhpVB could make VP28 directly interact with the shrimp when injected into shrim- p. In order to test the protection potential, live DhpVB were injected into the marine shrimp Exopalamon carincauda Holthuis for three times and WSSV challenge was performed twice simultaneously at the last two injections of the bacteria. The results showed that DhpVB displayed statistically significant advantage over bacteria without VP28 after the second challenge with an average RI （Resistance index） of 0.67±0.08, compared with 0.41±0.09 of bacteria without VP28 （P 〈0.05）. The data suggested that a quasi-immune response may exist in E. carincauda and live bacteria carrying VP28 could enhance the shrimp resistance against WSSV.
Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus （WSSV） envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a con- stitutive secretory expression plasmid as the expression vector. With the ability to deliver recombinant pro- tein products outside, DhpVB could make VP28 directly interact with the shrimp when injected into shrim- p. In order to test the protection potential, live DhpVB were injected into the marine shrimp Exopalamon carincauda Holthuis for three times and WSSV challenge was performed twice simultaneously at the last two injections of the bacteria. The results showed that DhpVB displayed statistically significant advantage over bacteria without VP28 after the second challenge with an average RI （Resistance index） of 0.67±0.08, compared with 0.41±0.09 of bacteria without VP28 （P 〈0.05）. The data suggested that a quasi-immune response may exist in E. carincauda and live bacteria carrying VP28 could enhance the shrimp resistance against WSSV.
基金
The National Basic Research Program of China (973 Program) under contract No. 2012CB114403
General Program of National Natural Science Foundation of China to Dr. Fuhua Li under contract No. 31072203
National Hightech Research and Development Program under contract Nos 2012AA10A404 and 2012AA092205
China Agriculture Research System under contract No. CARS-47
