摘要
Microtubule cytoskeleton plays an important role in cell division,differentiation,cell morphology decision,introcellular material transportation and other physiological activities.Performing those function depends on tubulin,microtubule-associated proteins(MAPs),correlated kinase and phosphate(ester) enzyme.In this article,GFP-TUA-6 and TUA-6-GFP gene were transferred into tobacco cotyledon mediated by Agrobacterium tumefaciens.Transformed lines were generated by the optimized genetic transformation system.It was proved that GFP-TUA-6 and TUA-6-GFP gene had been integrated into the tobacco genome by PCR detection and Southern blot analysis.The observation of laser scanning confocal microscope showed that GFP-TUA-6 protein was mainly located in cortical microtubules,while TUA-6-GFP protein was mainly located in cytoplasm and cortical microtubules,which provide a fundament for studying the dynamics activity of microtubule in cell morphogenesis.
Microtubule cytoskeleton plays an important role in cell division,differentiation,cell morphology decision,introcellular material transportation and other physiological activities.Performing those function depends on tubulin,microtubule-associated proteins（MAPs）,correlated kinase and phosphate（ester） enzyme.In this article,GFP-TUA-6 and TUA-6-GFP gene were transferred into tobacco cotyledon mediated by Agrobacterium tumefaciens.Transformed lines were generated by the optimized genetic transformation system.It was proved that GFP-TUA-6 and TUA-6-GFP gene had been integrated into the tobacco genome by PCR detection and Southern blot analysis.The observation of laser scanning confocal microscope showed that GFP-TUA-6 protein was mainly located in cortical microtubules,while TUA-6-GFP protein was mainly located in cytoplasm and cortical microtubules,which provide a fundament for studying the dynamics activity of microtubule in cell morphogenesis.
出处
《商丘师范学院学报》
CAS
2012年第6期71-77,共7页
Journal of Shangqiu Normal University
基金
supported by The Henan Province Science and Technology Development Program in China(grant no.092102310044)
The Ministry of Education Innovative Talent Supporting Program of Henan province in China(grant no.2010HASTIT019)
