姜黄素对人胃癌BGC-823细胞增殖及MMP-9、COX-2mRNA表达的影响

Modulating effects of curcumin on proliferation of gastric carcinoma cell line BGC-823 and the mRNA expressions of MMP-9 and COX-2

目的研究姜黄素在体外对人胃癌细胞BGC-823生长增殖的影响及其抗肿瘤的相关分子机制。方法体外培养人胃癌BGC-823,MTr法检测姜黄素在相同浓度下,不同时间对于胃癌细胞增殖的影响,并计算Ic蚰值;RT-PCR法检测其对COX-2、MMP-9mRNA表达的影响。结果MTlr结果显示:相同干预浓度姜黄素抑制人胃癌细胞增殖,其24、48、72h的IC_(50)分别为:(62.192±7.802)μmol/L、(46.365±8.991)μmol/L和(45.503±9.126)μmol/L;Rt—PCR结果显示:正常对照组及姜黄素干预组的人胃癌细胞BGC-823MMP-9mRNA灰度值分别为:0.704±0.045,0.147±0.052(P〈0.05);COX-2mRNA灰度值分别为:0.721±0.060,0.190±0.027(P〈0.05)。结论姜黄素抗肿瘤机制可能通过抑制COX-2及MMP-9mRNA的表达来抑制胃癌细胞增殖。

Objective To investigate modulating effects of curcumin on proliferation and the molecular mechanism in gastric carcinoma cell line BGC-823.Methods Ceils were incubated with curcumin in the same concentration for 24 h,48 h,and 72 h.The cell viability was measured by MTTassay.IC50 was calculated.RT-PCR was used to evaluate the expression of COX-2 and MMP-9.Results The BGC-823 cell viability was apparently inhibited by eurcumin with a inhibitory effect in the time-dependent and dosedependent manner.The IC_(50) of the BGC-823 cell line was(62.192±7.802)μmol/L(24 h),(46.365±8.991)μmol/L(48 h),and(45.503±9.126)μmol/L(72 h).RT-PCR showed the expressions of COX-2 and MMP-9 were down-regulated.The expression of MMP-9 mRNA in the BGC-823 cells was 0.704±0.045 for the normal control group and O.147±0.052 for the curcumin-treated group wit a statistical significance(P〈0.05);COX-2 were 0.721±0.060 for the normal control group and 0.190±0.027 for the curcumin-treated group with a statistical significance(P〈0.05).Conclusions The molecular mechanism that curcumin inhibited the proliferation of tumor cells might be that it inhibited preferentially the expressions of COX-2 and MMP-9 in gastric carcinoma cell line.