可可西里-唐古拉山冻土区沉积物样品可培养微生物的研究

Bacteria Isolated from Soil Samples of Hoh Xil-Tanggula Mountains

地壳运动引起的高海拔使得青藏高原成为一个独特的高原永久冻土环境,该环境在微生物的分类和多样性上占有重要的地位。青藏高原冻土区表层的活动层并非永久冻土环境,但是冻土活动层却构成了冻土环境中的一个重要组成部分。本文中选取了青藏高原可可西里-唐古拉山冻土区达哈煤矿附近的两个土壤样品作为材料进行研究。分别在LB和PYGV培养基上进行微生物的培养,总共得46株纯培养,并得出在两种培养基上的CFU在0～6×107/g土壤之间。在这46株被发现的菌株中,通过16SrDNA和系统发育树分析得出,这些菌株属于:放线菌门、厚壁菌门、变形菌门,其中变形菌门和放线菌门占优势地位,并用基因序列比对的方法分析了该地区的可培养微生物的分类及与已知菌种相似性。

The Qinghai-Tibet Plateau represents an unique and important permafrost environment as a result of the high altitude caused by earth movements. It is known that mobile layer which is not permafrost in surface layer of permafrost region is an es- sential part. In this study,two soil samples for research were collected from the sites nearby Daha coal mine of the permafrost region in Hoh Xil and Tanggula mountains,46 bacterial strains in pure culture were obtained in LB and PYGV culture medium with 0 ~ 6 X 107/g CFU. By means of 16SrDNA and phylogenetic tree analysis,it was found that 46 bacterial strains belonged to three phyla： Aetinobacteria, Firmieutes and Prote^3baeteria, among these strains, Actinobaeteria and Proteohacteria were discov- ered having the dominant position. Furthermore,through the method of gene sequence comparison, this paper also analysed the classification of culturable microorganisms and similarity of known strains in the sample region.