应用组织芯片检测新疆哈萨克族食管癌核黄素转运基因2蛋白表达及意义

Detecting the Expression of Human Riboflavin Transporter 2 in Xinjiang Kazakh Esophageal Squamous Cell Carcinoma Using Tissue Chip Technology and its Clinical Significance

为检测新疆哈萨克族(哈族)食管鳞癌中核黄素转运基因2(hRFT2)蛋白表达,以探讨hRFT2蛋白在新疆哈族食管鳞癌中的意义。应用组织芯片技术结合免疫组织化学Envision法染色,检测新疆哈族石蜡包埋食管鳞癌组织119例及60例癌旁非癌组织中hRFT2蛋白表达。结果显示,(1)60例哈族癌旁非癌组织中,61.7%hRFT2蛋白细胞核阳性表达伴有少量细胞浆阳性的表达,38.3%阴性表达;在119例食管鳞癌组织中,95.0%出现细胞浆或细胞膜的弥散阳性表达,其余5.0%阴性;(2)食管鳞癌高分化、中分化及低分化三组间hRFT2蛋白表达的阳性率有逐渐降低的趋势,但其三组间hRFT2蛋白表达差异没有统计学意义(P>0.05);(3)按发病年龄、性别、临床TNM分期与淋巴结转移等临床病理特征分组的新疆哈族食管鳞癌中,hRFT2蛋白表达差异没有统计学意义(P>0.05)。由此可知,在新疆哈族食管鳞癌中hRFT2蛋白的表达异常,主要表现为蛋白从细胞核到细胞浆/膜的转位,其异常表达可能与食管癌的发生有关。

To investigate the expression of human riboflavin transporter 2 （hRFT2）, and its relationship with esophagus squamous cell carcinoma （ESCC）. Methods： Using tissue chip and immunohistochemical Envision method, we selected 119 ESCC cases and 60 non-can- cerous adjacent tissues（NCAT ）, and all of them were paraffin-embedded. （1）In 60 cases NCAT, the expression of hRFT2 was found 37 （61.7 % ） in nucleus and little cytoplasm positive, other 23 （38.3 % ） showed negative And in 119 ESCC, i 13 （ 95 % ） showed cytoplasm positive,other 6 （5 %）showed negative. （2）Among the three groups（good, moderate and poor differentiation）, the expression rates of hRI＂T2 were % 96.7 %, 96.0 %, 85.7 %, respectively; （ 3 ） The expression of hRFT2 in ESCC didn＇t correlate with sex, age, TNM stage and lymph node metastasis（P〈0.05）. The expression of hRVI＇2 in Xinjiang Kazakh ESCC is abnormal,mainly including the transloca- tion from nucleus to cytoplasm/membrane,which may relate to the carcinogenesis of ESCC.