5种沙漠微藻的分离鉴定及其18S rDNA保守区片段差异分析

Isolation and Identification of Five Desert Microalgae and Analysis of 18S rDNA Conserved Fragment

为了研究古尔班通古特沙漠中微藻的种类,采用96孔板有限稀释法,从该沙漠采集的沙样中分离出5种不同的微藻,并通过形态学特征初步鉴定为绿藻门。根据GenBank中绿藻门不同科属藻类的18S核糖体RNA基因(18S rDNA)保守区设计18S特异性引物,分别对5种微藻的基因组进行PCR扩增18S rDNA片段,经克隆测序、blast n比对、系统发育树构建及遗传距离值分析,将5种微藻初步鉴定到属。结果表明:从该沙漠分离得到的5个微藻物种分别来自绿藻门不同的科属,其中一个物种极可能为新物种。

To study microalgae species of Gurbantunggut Desert,this experiment isolated five desert microlgae from the sand samples by using the method of 96-well plate limited dilution.The isolated microalgae were preliminarily identified morphologically among Chlorophyta.The specific gene primers of 18S ribosomal RNA gene were designed based on the conserved region of 18S rRNA gene sequences available in the GenBank from different green algae,and the 18S rRNA gene sequences were amplified. After nucleotide blast analysis,the phylogenetic tree construction and genetic distance calculation,the isolated species were identified to the genus preliminarily.The results show that the isolated species belong to different genus,and one of them is very likely to be a new species.