抗犬瘟热高免卵黄抗体的制备及效价的检测

Preparation and Titer Detection of Immunoglobuin of Yolk against Canine Distemper

为了制备出抗犬瘟热高免卵黄抗体并进行抗体效价检测,以便为抗犬瘟热高免卵黄抗体用于患病犬的临床治疗奠定基础。本研究用自行设计的免疫程序对健康产蛋鸡用犬瘟热和犬细小病毒二联疫苗进行免疫,同时使用卡介苗和白介素-16(IL-16)作为佐剂,用石河子地区患犬瘟热的病犬做组织灭活苗对产蛋鸡进行了强化免疫注射,用IHA(间接血凝)方法进行了卵黄抗体检测,对检测出效价最高的批次用盐析法粗提制备了抗犬瘟热卵黄抗体。结果显示:从疫苗开始注射后第20-102天收集的鸡蛋,其卵黄抗体效价均在1:64以上,可以用于临床治疗,其中第77-84天卵黄抗体效价最高(1:8192)。结论:本研究设计的免疫程序合理,用IHA方法检测出的高效价卵黄抗体可以用盐析法制备出抗犬瘟热高免卵黄抗体。

To prepare IgY against Canine Distempe and to detect the titers,which can provide a base for clinical treatment of sick dogs.In this study,laying hens were immunized by Canine Distemper and canine parvovirus bivalent vaccine by self-designed program,using BCG and interleukin-16（IL-16） as adjuvants.The tissue of distemper dogs in Shihezi were used for inactived vaccine,which were immunized to laying hens.IHA method was used to detect egg IgY.The highest titer was used to prepare IgY against Canine Distemper by crude salt extraction.The results showed that,after injection,the IgY titers of eggs collected from 20-102 d can be used for clinical treatment,the highest antibody titers occurred in eggs between 77-84 d（1：8192）.Conclusion： The immunization program this paper designed is reasonable.IgY detected by IHA method can be used to prepare hyperimmuned Immunoglobuin of Yolk against Canine Distemper.