银杏抗寒优良无性系选育

Selection and breeding of Ginkgo cold-resistant fine clones

采用-20℃低温处理方法 ,分析了39个银杏无性系枝条的LT50,可溶性糖含量、可溶性蛋白含量,SOD活性等指标的变化。运用多元统计主成分分析,初步判断受试材料抗寒性,由强到弱排序为:16#、38#、17#、40#、41#、36#、45#、15#、53#、44#、19#、42#、57#、10#、35#、18#、50#、55#、32#、26#、12#、30#、56#、9#、13#、34#、12#、37#、14#、33#、54#、20#、28#、11#、31#、27#、29#、39#。其中抗寒性较高的银杏无性系有16#、41#、36#、45#、19#、17#、38#、40#,表现为较好的保水能力、抗氧化能力和渗透调节能力,从而表现出较强的抗寒力,具有在高纬度或高海拔地区推广试验的潜力。

By using -20 ℃ low temperature processing methods, the changes of semilethal temperature, soluble sugar, soluble protein contents, SOD activity and others of 12 indexes measured for 39 Ginkgo clones branches were analyzed. The cold resistant performance of the tested materials were sorted from strong to weak ： 16#,38#,17#,40#,41#,36#,45#,15#,53#,44#,19#,42#,57#,10#,35#,18#,50#,55# ,32#,26#,12#,30#,56#,9#, 13#,34#,12#,37#, 14#,33#,54#,20#,28#, 11#,31 #,27#,29# and 39#. Of them, the clones having relatively stong cold resistant were 16#,41#,36#,45#,19#,17#,38# and 40#, they had good water retaining capacity, antioxidant capacity and osmotic regulation ability, thus they showed a relatively stong cold resistant, and have the potentiality of popularizing and planting in high latitudes or high altitude areas.