Optimization of the Extraction Process of Polysaccharides from Tegillarca granosa by Response Surface Methodology

[ Objective] The aim was to use response surface methodology to determine optimum conditions for extraction of polysaccharides from Tegillarca granosa. [ Method] Response surface methodology with three-factors and throe-levels was carried out for optimizing the extraction process of polysacchafides from Tegillarca granosa. A central composite des（gn including independent variables, such as extraction temperature （A）, extraction time （B）, and ethanol concentration （C） was obtained through Box-Benhnken central combination design. Selected response which evaluates the extraction process was polysacchadde yield. [ Result] The independent variable with the largest effect on response was ethanol concentration （C）. The optimum extraction conditions were found to be extraction temperature 69.6℃, extraction time 6.2 h, and ethanol concen- tration of 78% （V/V）, respectively. Under these conditions, the extraction efficiency of polysaccharide can increase to 1. 635%. [ Coaclusioa] Study on the extraction of polysaccharides from Tegillarca granosa could provide certain theoretical direction for extracting polysaccharides from Tegillarca granosa on a large scale.

[Objective] The aim was to use response surface methodology to determine optimum conditions for extraction of polysaccharides from Tegillarca granosa. [Method] Response surface methodology with three-factors and three-levels was carried out for optimizing the extraction process of polysaccharides from Tegillarca granosa. A central composite design including independent variables, such as extraction temperature (A), extraction time (B), and ethanol concentration (C) was obtained through Box-Benhnken central combination design. Selected response which evaluates the extraction process was polysaccharide yield. [Result] The independent variable with the largest effect on response was ethanol concentration (C). The optimum extraction conditions were found to be extraction temperature 69.6 ℃, extraction time 6.2 h, and ethanol concentration of 78% (V/V), respectively. Under these conditions, the extraction efficiency of polysaccharide can increase to 1.635%. [Conclusion] Study on the extraction of polysaccharides from Tegillarca granosa could provide certain theoretical direction for extracting polysaccharides from Tegillarca granosa on a large scale.