依达拉奉联合还原型谷胱甘肽对百草枯中毒小鼠肺纤维化的影响

The effect of edaravone companied with glutathione in pulmonary fibrosis induced by edaravone companied with glutathione in mice

目的观察联合应用依达拉奉和还原型谷胱甘肽对小鼠百草枯中毒引起的肺纤维化的干预作用。方法昆明小鼠90只,随机分为空白对照组(N组)、急性百草枯中毒组(P组)、单独依达拉奉治疗组(E组)、单独谷胱甘肽治疗组(G组)、两种药物联合治疗组(L组)。除空白对照组外,其余组均以百草枯(80 mg/kg)灌胃染毒,灌胃后实验组分别用依达拉奉(3 mg/kg),或(和)谷胱甘肽(300 mg/kg),尾静脉注射。各组分别于中毒后第1、7、14天取其肺脏。通过HE染色观察肺组织病理变化,免疫组化染色观察MMP-2和MMP-9的表达,利用羟脯氨酸试剂盒检测肺组织匀浆内羟脯氨酸含量。结果 E、G和L组与P组相比,肺纤维化进展相对缓慢,程度均减轻,L组改善效果最明显。E、G和L组与P组相比,肺组织中MMP-2和MMP-9的表达均降低,以L组差异最显著,其差异具有统计学意义(P<0.05)。结论依达拉奉和还原型谷胱甘肽联合应用可明显减轻肺水肿、肺充血和肺出血等肺泡炎的病理过程,最终抑制并延缓肺纤维化的发生。

Objective The effect of edaravone companied with glutathione in pulmonary fibrosis induced by edaravone companied with glutathione in mice. Methods Ninety male Kunming mices were randomized to five groups ： Normal controlled group, acute paraquat intoxication model group, Edaravone therapy group, Glutathione therapy group, and Edaravone companied with glutathione therapy group. The pathological changes of lungs of those mices were examined by microscope, and the intensity of expression of MMp-2 and MMP-9 was detected by immunohistochemistry in the 7 th and 14 th days. Results The degree of pulmonary fubrosis of the group with edaravone companied with glutathione therapy is the lowest. The intensity of expression of MMp-2 and MMp-9 was significantly higher in the mites of acute paraquat intoxication model comparied with the control group （ P〈0：05 } through the study. The intensity of expres- sion of MMp-2 and MMp-9 was highest in the mices of acute paraquat intoxication model group （ P〈0. 05 } through the study. Conclu- sion Edaravone companied with glutathione may have a role in preventing the development of lung injury and pulmonary fibrosis in- duced by acute paraquat intoxication.