联合检测EMs患者腹腔液中瘦素及IL-8的临床意义

Analyzing levels of both leptin and IL-8 in peritoneal fluid in women with endometriosis

目的通过联合检测EMs患者腹腔液中瘦素及IL-8的表达水平,分析二者与EMs疾病分期、分型及临床表现的相关性,探讨二者在EMs发病中的作用。方法收集46例EMs患者及31例非内异症对照病例的腹腔液,采用ELISA法定量检测两组患者腹腔液中瘦素及IL-8的含量,分析瘦素、IL-8与EMs疾病分期、分型及临床表现的相关性。结果瘦素、IL-8与EMs组患者年龄、体重、身高、体重指数及孕次均无相关性。EMs患者腹腔液中瘦素、IL-8的表达水平与对照组比较,差异有统计学意义(P<0.01)。瘦素在早期EMs患者腹腔液中的表达水平与晚期EMs组比较,差异有统计学意义(P<0.01),而IL-8在晚期EMs患者腹腔液中的表达水平与早期EMs组比较,差异有统计学意义(P<0.05);瘦素与rFAS分期负相关,IL-8与rFAS正相关;晚期EMs患者IL-8表达水平与痛经/慢性盆腔疼痛评分正相关;腹膜内异灶组的瘦素表达高于卵巢异位囊肿组。结论 EMs组患者腹腔液中瘦素及IL-8异常增高,与炎症和免疫调节异常有关。瘦素与EMs的早期发病相关;IL-8可能以剂量依赖的方式促进了EMs的进展。

Objective To compare levels of leptin and IL-8 in peritoneal fluid in women with endometriosis, and to explore the two angiogenic cytokines for a possible role in the pathophysiology of endometriosis. Methods Peritoneal fluid from 46 women with endometriosis and 31women undergoing hysteromyoma eetomy was coUeeted at lapanrocopy. Leptin and IL-8 levels were detected using ELISA. The correlation of letin and IL-8 in stage, rFAS, type and presentation of endometriosis was analyzed. Results Presence of age, weight, height, BMI and gravidity did not show any significant correlation with both leptin and IL-8. Significantly higher leptin and IL- 8 were demonstrated in peritoneal fluid in patients with endometriosis comparing with control （P 〈 0. 01 ）. Levels of leptin were signifi- candy higher in early stage than advanced stage{ P 〈 0. 05 }, Contrary to leptin, IL-8 levels were significandy elevated in those with ad- vanced disease{ P 〈 0. 05 }. Levels of leptin correlated negatively with rFAS, and levels of IL-8 correlated positively with rFAS. Pain scores were positively correlated with levels of IL-8 in women with advanced endometriosis. Women with peritoneal implants had signifi- cantly higher IL-8 levels than those with endometriotie cysts. Conclusion Leptin and IL-8 levels are raised in peritoneal fluid of women with endometriosis, which reflects inflanlmation and dysregulated immunomodulation. Higher levels of leptin were correlated with early stages, and IL-8 seems to stimulate endometriosis aggravation in a dose-dependent manner.