亚硒酸钠拮抗氯化镉染毒大鼠肝、肾细胞损伤的研究

Protective Effects of Sodium Selenite against Cadmium Chloride-Induced Injury on Kidney and Liver Cells of Rat

目的观察亚硒酸钠处理对亚慢性氯化镉中毒大鼠肝、肾结构的影响,为氯化镉慢性中毒的防治和亚硒酸钠蓄积性毒性研究提供依据。方法雄性SD大鼠54只,随机分成3组。对照组大鼠饮用蒸馏水;其余两组大鼠饮用含30 mg/L氯化镉蒸馏水;硒干预组大鼠给予5μmol/kg亚硒酸钠溶液灌胃。结果与对照组比较,氯化镉组大鼠组织中GSH-Px及SOD活性降低、MDA含量增加;硒干预组大鼠组织GSH-Px及SOD活性显著升高、MDA含量降低。组织病理切片观察结果,单纯给氯化镉组大鼠肾小球肿胀或固缩,肾小囊囊腔增大,肾小管上皮细胞变性坏死并脱落等病理损伤。硒干预组大鼠肝、肾脏组织结构未见明显损伤。结论饮用含氯化镉的水源可致肾脏组织明显损伤,低剂量亚硒酸钠可拮抗氯化镉对肾组织的损伤作用,对氯化镉所致组织氧化损伤有一定的修复作用。

Objective To study the -induced injury on kidney and liver cells of rat and the protective effects of sodium selenite. Methods 54 healthy SPF male SD rats were divided randomly into three groups. Group I ：rats drinking distilled water as control; Group Ⅱ ：rats drinking water containing 30mg/L CdC12 distilled water; Group Ⅲ ：rats drinking water containing 30mg/L CdC12 distilled water, while giving 5μmoL/kg Na2SeO3solution garage, 5 days a week, once a day. Rats were killed on the 2nd, 4th and 8th week after initiation of the experiment. Blood were collected, kidneys and livers were removed immediately, ？ xed in 10% methanal ？ xative, routinely processed and stained with hematoxylin and eosin. Results In Se - administered rat homogenates, the contents of MDA were lower, and the activity of GSH - Px and SOD were significantly higher than those of control. In Cd - treated rats, we noticed histopathological changes in kidneys and livers, while no obvious changes were found in Se - administered rats. Conclusion The sodium selenite could efficiently protect the - induced injury on kidney and liver cells of rat.