脑脊液14-3-3蛋白与吉兰-巴雷综合征病程关系的研究

Relationship between 14-3-3 protein and process of Guillain-Barre Syndrome

目的观察吉兰-巴雷综合征(GBS)患者脑脊液(CSF)中14-3-3蛋白如何随病程变化。方法采用酶联免疫吸附试验法(ELISA)检测45例GBS患者起病后3 d内、第1周、第2周、第4周、第12周的脑脊液14-3-3蛋白含量,观察脑脊液14-3-3蛋白含量变化与病程的关系。结果 GBS组在起病3 d内、第1周、第2周、第4周、第12周的脑脊液14-3-3蛋白含量分别为(6.312±2.677)ng/mL、(8.639±3.544)ng/mL、(14.351±6.721)ng/mL、(9.533±3.426)ng/mL、(6.168±2.652)ng/mL,GBS组脑脊液14-3-3蛋白第1周、第4周高于对照组,差异有显著性(P<0.05),第2周明显高于对照组,差异有显著性(P<0.01)。结论 GBS患者起病早期脑脊液14-3-3蛋白含量低,随病程进展逐渐升高,恢复期逐渐降至正常。

[Objective] To observe the variation of 14-3-3 protein during the process of Guillain-Barre Syndrome. [Methods] ELISE KIT was employed to measure the amount of 14-3-3 protein of the cerebrospinal fluid in 45 patients with Guillain-Barre Syndrome of onset within 3 days, on the 1st week and the 2nd week and the 4th week and the 12th week, to observe the relation between the variation of 14-3-3 protein and process of Guillain- Barre Syndrome. [Results] The concentration of 14-3-3 protein in the cerebrospinal fluid within 3 days, on the 1st week and the 2nd week and the 4th week and the 12th week were （6.312±2.677）ng/mL, （8.639±3.544）ng/mL, （14.351±6.721）ng/mL, （9.533±3.426）ng/mL, （6.168±2.652）ng/mL, the 14-3-3 protein increased on the 1st week and the 4th week as compared to that of the control group （P 〈0.05） and significantly higher on the 2nd week as com- pared to that of the control group （P 〈0.01）. [Conclusions] In the early stage of Guillain-Barre Syndrome, the amount of 14-3-3 protein was low, it increased following process of illness and decreased to normal in the recovering period of illness.