调节性T细胞与慢性移植物抗宿主病关系的研究

Chronic graft-versus-host disease is associated with increased numbers of peripheral blood regulatory T cells

目的研究自调节性T细胞在同种异基因造血干细胞移植(allo-HSCT)中对慢性移植物抗宿主病(cGVHD)的影响,以及其发生机制。为慢性移植物抗宿主病的早期诊断及预后提供检验依据,指导临床上免疫调节剂等药物的应用。方法回顾性研究广东省人民医院2002年4月-2010年8月62例造血干细胞移植植入成功患者,其中42例发生cGVHD,分别采用免疫细胞流式细胞仪和全自动五分类血常规分析仪检测T细胞表型和T细胞计数,研究无cGVHD组与cGVHD组间T细胞的百分比和绝对数是否存在差异,以及不同分级状态下cGVHD组间的T细胞百分比和绝对数的动态变化。结果 cGVHD患者中T细胞的百分比和绝对数明显升高(P<0.05)。不同分级状态下,随着cGVHD分级程度的加重,T细胞的百分比和绝对数均表现出升高的分布趋势。结论 T细胞在cGVHD的发生、发展中起重要作用。

[ Objective ] To explore the effect of T lymphocytes on graft-versus-host disease （GVHD） after allo- geneic hematopoietic stem cell transplantation （allo-HSCT）. [ Methods ] The 42 patients who suffered of hematology diseases or malignant solid tumors and were underwent allo-HSCT and 10 normal individuals were enrolled in the study. 62 patients were enrolled in the Guangdong General Hospital from April 2002 to August 2010. All patients received hematopoietic stem cell transplantation. Incidence of graft-versus-host disease were found in 42 cases, T- cell phenotype and T-cell count, were detected by flow cytometry and automatic blood analysis respectively. [Resuits] We investigated the number and function of peripheral blood CD4^＋CD25^＋-T cells in patients more than 180 days after allogeneic hematopoietic stem cell transplantation transplantation. Patients with cGVHD had markedly el- evated numbers of CD4^＋CD25^＋-T cells as compared to patients without GVHD. [ Conclusion ] A population of CD4^＋CD25^＋-T cells has been characterized, which controls immunologic reactivity and which on transfer can prevent acute GVHD.