无机阳离子对酪氨酸酶活性的上调作用

Upraising of inorganic cationic on the activity of tyrosinase

考查了无机阳离子Cu2 + 、Zn2 + 、Mg2 + 、Ca2 + 对酪氨酸酶活性的影响。结果显示上述无机阳离子对酪氨酸酶的影响不仅与离子的种类有关 ,而且还与其浓度有关 ;在 1 .5× 1 0 -5 mol/L时无机阳离子对酪氨酸酶活性的影响 ,分别是Cu2 + 激活率达 1 6 8%、Zn2 + 激活率达 6 8.1 %、Mg2 + 激活率达 33.7%、Ca2 + 激活率达 40 .6 % ;而当浓度超过一定范围时 ,上述离子对酪氨酸酶有抑制作用。Cu2 + 、Mg2 + 、Zn2 + 对酪氨酸酶具有竟争性激活作用 ,Ca2 + 对酪氨酸酶具有非竟争性激活作用。

This paper describes the experimental study for the effects of inorganic cationic Cu 2+ 、Zn 2+ 、Mg 2+ and Ca 2+ on the activity of tyrosinase.The results reveal that the effects are not only associated the kind of ions, but alsorelevant to their concentrations. At the concentration 1×10 -5 mol/L,the activation ratio of Cu 2+ was 168%,that of Zn 2+ 、Mg 2+ and Ca 2+ respectively are 68.1%,33.7%,40.6%. When the concentrations are beyond the normal range, the inorganiccationic shows inhibitory effects. Cu 2+ 、Mg 2+ 、Zn 2+ possesscompetitive activating effects on tyrosinase, while Ca 2+ is noncompetitive.