G-CSF对大鼠烧伤合并海水浸泡后内皮祖细胞的影响

Effects of granulocyte colony-stimulating factor on the number of circulating endothelial progenitor cells in burned rats with seawater immersion

目的观察早期应用粒细胞集落刺激因子(G-CSF)对大鼠烧伤合并海水浸泡后血液中的内皮祖细胞(endothe-lial progenitor cells,EPC)的影响。方法将26只SD大鼠随机分成3组:对照组、烧伤合并海水浸泡模型组和烧伤合并海水浸泡+G-CSF实验组。在模型制备后的30 min、2 h、6 h和24 h,使用双色荧光标记流式细胞术检测不同组别大鼠的血中EPC数量。结果烧伤合并海水浸泡使血中EPC的数量显著降低,G-CSF可以部分逆转烧伤合并海水浸泡对EPC数量减少的作用。结论在烧伤合并海水浸泡情况下,使用G-CSF可增加血中EPC数量,并可能增强创面的修复能力。

Objective To study the influence of application of granulocyte colony-stimulating factor （G-CSF） on the number of circulating endothelial progenitor cells （EPC） in burned rats with seawater immersion. Methods Rats were randomly divided into three groups：normal control group,bums ＋ seawater immersion model group and bums ＋ seawater immersion ＋ G-CSF group. The blood was harvested and the peripheral mononuclear blood cells were isolated by density gradient centrifugation using Ficol. The number of EPCs was determined using flow cytometry with FITC-Sca-1 and PE-VEGFR2 at four time points （30 minutes,2 hours,6 hours and 24 hours）. Results Bum plus seawater immersion could significantly reduce the number of EPC in the blood. In contrast, G-CSF partly reversed this phenotype. Conclusion The G-CSF may be a promising agent in treating bum and seawater immersion.