益气固本胶囊调节脾气虚哮喘模型小鼠脾淋巴细胞体外增殖及相关脏器指数的实验研究

Experimental research into Yiqi Guben capsule in regulating the spleen lymphocyte in vitro proliferation and related organ exponent in the spleen-Qi deficiency asthma model mice

目的观察益气固本胶囊对脾气虚哮喘小鼠脾淋巴细胞体外增殖及脏器指数的影响并探讨其意义。方法将96只昆明种小鼠随机分成7组,除哮喘组12只外,其余每组14只,雌雄各半。正常对照组、哮喘组、脾气虚哮喘组、适贝高儿宝颗粒组、益气固本胶囊低、中、高剂量组。采用淋巴细胞转化实验测定脾淋巴细胞体外增殖功能;取脾、胸腺、肾称重,计算其脏器系数并比较各组间的差异。结果益气固本胶囊低、中、高剂量组及适贝高儿宝颗粒组小鼠胸腺质量增加不显著(P>0.05);益气固本胶囊中、高剂量组及适贝高儿宝颗粒组小鼠由脂多糖刺激的脾淋巴细胞增殖明显高于脾气虚哮喘组,差异有统计学意义(P<0.05)。结论益气固本胶囊能明显改善脾气虚哮喘小鼠脾气虚哮喘的状态,提高脾淋巴细胞增殖功能。

Objective To observe the impact of Yiqi Guben capsule on the splenic lymphocyte in vitro proliferation and organ exponent of spleen-Qi deficiency asthma mice and study its significance. Method Totally 96 mice were randomly divided into 7 groups, apart from the asthma group consisting of 12 mice, the remaining groups each consisted of 14 mice（male and female in half）. They were normal control group, asthma group, spleen-Qi deficiency asthma group, Shibeigao-erbao granule group, the Yiqi Guben capsule low-close group, middle-close group and high-close group. Lymphocyte transformation test was used to measure the function of splenic lymphocyte in vitro proliferation; the spleen, thymus, and kidney were taken and weighed, the organ exponent was calculated and the differences were compared between each group. Result The mice＇s thymus weight in the Yiqi Guben capsule low-close group,middle-dose group, high-dose group ancl Shibeigao-erbao granule group did not increase significantly; the spleen lymphocyte proliferation stimulated by lipopolysaccharide in Yiqi Guben capsule middle-dose group, high-dose group and Shibergao-erbao granule group was significantly higher than the spleen-Qi deficiency asthma group（P〈0.05）. Conclusion Yiqi Guben capsule can significantly improve the asthma state of spleen-Qi deficiency asthmatic mice, and improve the function of spleen lymphocyte proliferation.