期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

马拉色菌培养基的改良及应用 被引量：4

Improvement and application of culture medium for malassezia species
下载PDF
导出
摘要 目的改良传统的Leeming&Notman培养基,提高马拉色菌的培养效率,并实际应用。方法设立含1%橄榄油、2%橄榄油和4%橄榄油的培养基组。在培养第3、5、7日测量菌落直径,提取基因组DNA并检测浓度,扩增ITS1-2区比较PCR产物质量。结果马拉色菌在含4%橄榄油的培养基上生长最为迅速,产出的基因组DNA量最大,扩增得到的ITS1-2区产物质量最佳。结论改良的含4%橄榄油的Leeming&Notman培养基有利于提高马拉色菌科研工作的效率。 Objective To improve the efficiency of traditional Leeming＆Notman culture medium for Malassezia species and put into practical application. Methods Three groups of culture mediums containing 1% ,2% or 4% of olive oil were established for a- nalysis. On the 3rd,5th and 7th day of culturing, the diameter of each colony was measured, the genomic DNA of Malassezia isolates was extracted for quantitative analysis, and the ITS1-2 region was amplified for quality evaluation. Results Malassezia isolates on 4% of ol- ive oil medium grew most rapidly, yielded the greatest amount of genomic DNA, and guaranteed best PCR products of ITS1-2 region. Conclusion Modified culture medium containing 4% of olive oil for Malassezia species is likely to improve the efficiency of research work.
作者 穰真 崔凡 李薇 王有为
机构地区 四川大学华西医院皮肤性病科 四川省医学科学院.四川省人民医院皮肤病性病研究所
出处 《实用医院临床杂志》 2013年第2期31-33,共3页 Practical Journal of Clinical Medicine
基金 四川省卫生厅科研基金资助项目(编号:080328)
关键词 马拉色菌属 Leeming&Notman培养基 真菌培养 橄榄油 Malassezia species Leeming＆Notman medium Fungal culture Olive oil
分类号 R446.5 [医药卫生—诊断学] R379 [医药卫生—病原生物学]
  • 相关文献

参考文献8

  • 1Midgley G. The lipophilic yeasts : state of the art and prospects [ J ]-Med Mycol,2000,38(Suppl 1) :9-16.
  • 2Benham R W. The cultural characteristics of Pityrosporum ovale-ali-pophilic fungus[ J]. J Investig Dermatol, 1939,2 : 187-203.
  • 3van Belkum A,Boekhout T,Bosboom R. Monitoring spread of Malas-sezia infections in a neonatal intensive care unit by PCR-mediatedgenetic typing[ J]. J Clin Microbiol,1994,32:2528-2532.
  • 4Guillot J,Gueho E,Lesourd M. Identification of Malassezia species:apractical approach[ J ]. J Mycol Med, 1996,6 : 103-110.
  • 5崔凡,陶诗沁,沈永年,吕桂霞,陈伟,李筱芳,胡素泉,杨丽佳,刘维达.马拉色菌临床株分类鉴定的研究[J].中华皮肤科杂志,2005,38(8):492-494. 被引量：11
  • 6Aizawa T, Kano R, Nakamura Y, et al. Molecular heterogeneity inclinical isolates of Malassezia pachydermatis from dogs[ J ]. Veterina-ry Microbiology,1999,70( 1-2) :67-75.
  • 7Roberts SOB. Pityrosporum orbiculare;incidence and distribution onclinically normal skin[ J] . Br J Dermatol, 1969,81 :264-269.
  • 8Nakabayashi A,Sei Y,Guillot J. Identification of Malassezia speciesisolated from patients with seborrhoeic dermatitis, alopic dermatitiss,pityriasis versicolor and normal subjects [ J ] . Medical Mycology,2000,38:337-341.

二级参考文献8

  • 1Belkum AV, Boekhout T, Bosboom R. Monitoring spread of Malassezia infections in a neonatal intensive care unit by PCR- mediated genetic typing. J Clin Microbiol, 1994, 32: 2528-2532.
  • 2Nakabayashi A, Sei Y, Guillot J. Identification of Malassezia species isolated from patients with seborrhoeic dermatitis, atopic dermatitis,pityriasis versicolor and normal subjects. Med Mycol, 2000, 38:337-341.
  • 3Velegraki A, Kambouris M, Kostourou A, et al. Rapid extraction of fungal DNA from clinical samples for PCR amplification. Med Mycol, 1999, 37: 69-73.
  • 4Guillot J, Gueho E. The diversity of Malassezia yeasts confirmed by rRNA sequence and nuclear DNA comparisons. Antonie Van Leeuwenhoek, 1995, 67: 297-314.
  • 5Gupta AK, Kohli Y, Summerbell RC. Molecular differentiation of seven Malassezia species. J Clin Microbiol, 2000, 38: 1869-1875.
  • 6Gaitanis G, Velegraki A, Frangoulis E, et al. Identification of Malassezia species from patient skin scales by PCR-RFLP. Clin Microbiol Infect, 2002, 8: 162-173.
  • 7Midgley G. The fipophilic yeasts: state of the art and prospects. Med Mycol, 2000, 38(Suppl 1 ): 9-16.
  • 8熊琳,冉玉平,周光平,代亚林.马拉色菌属的分类及其在几种皮肤病的分布情况[J].中华皮肤科杂志,2000,33(5):318-320. 被引量：53

共引文献10

同被引文献66

  • 1Williams HC. Epidemiology of human atopic dermatitis - - seven areas of notable progress and seven areas of nota- ble ignorance[ J ]. Vet Dermatol, 2013,24 ( 1 ) : 3-9, e1- e2.
  • 2Sugita T, Suto H, Unno T, et al. Molecular analysis of Malassezia microflora on the skin of atopic dermatitis pa- tients and healthy subjects[ J]. J Clin Microbiol, 2001,39 (10) :3486-3490.
  • 3Zhang E, Tanaka T, Tajima M, et al. Anti-Malassezia- specific IgE antibodies production in Japanese patients with head and neck atopic dermatitis: relationship be- tween the level of specific IgE antibody and the coloniza- tion frequency of cutaneous Malassezia species and clini- cal severity[ J]. J Allergy ( Cairo), 2011,2011:645670.
  • 4Back, Bartosik J. Systemic ketoconazole for yeast allergic patients with atopic dermatitis [ J 1. J Eur Acad Dermatol Venereol, 2001, 15(1) :34-38.
  • 5Johansson C, Ahlborg N, Andersson A, et al. Elevated peripheral allergen-specific T cell response is crucial for a positive atopy patch test reaction [ J ]. Int Arch Allergy Immunol, 2009,150( 1 ) :51-58.
  • 6Lind SM, Kuylenstierna C, Moll M, et al. IL-18 skews the invariant NKT-cell population via autoreactive activa-tion in atopic eczema[ J]. Eur J Immunol, 2009,39 (8) : 2293-2301.
  • 7Johansson C, Sandstrom MH, Bartosik J, et al. Atopy patch test reactions to Malassezia allergens differentiate subgroups of atopic dermatitis patients [ J ]. Br J Derma- tol, 2003,148 (3) :479-488.
  • 8Hiragun T, Ishii K, Hiragun M, et al. Fungal protein MGL_1304 in sweat is an allergen for atopic dermatitis patients [ J ]. J Allergy Clin Immunol, 2013,132 (3) : 608-615.
  • 9Ishibashi Y, Kato H, Asahi Y, et al. Identification of the major allergen of Malassezia globosa relevant for atopic dermatitis [ J ]. J Dermatol Sci, 2009, 55 ( 3 ) : 185-192.
  • 10Svejgaard El, Larsen PO, Deleuran M, et al. Treatment of head and neck dermatitis comparing hraconazle 200 mg and 400 nag daily for 1 week with placebo[ J]. J Eur Acad Dermatol Venereol, 2004,18 (4) :445- 449.

引证文献4

二级引证文献7

实用医院临床杂志
2013年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部