B亚群禽白血病病毒SDAU09C2株的TCID_(50)与p27抗原之间的相关性研究

Study of the Correlation between TCID_(50) and p27 Antigen of Subgroup B Avian Leukosis Virus

为了研究B亚群禽白血病病毒(ALV-B)的半数细胞培养物感染量(TCID50)与p27抗原的酶联免疫吸附试验(ELISA)的S/P值之间的相关性,本试验将ALV-B SDAU09C2株接种鸡胚成纤维细胞(CEF细胞),换维持液后连续10d取样,检测10d的TCID50值与p27抗原的S/P值之间的相关性;同时,将该毒株在DF-1细胞系上传代至20代,取其中的第1、5、10、15和20代分别进行TCID50滴度的测定和p27抗原检测。结果表明,在CEF细胞上接种的ALV-B SDAU09C2株连续10d的TCID50值与p27抗原之间存在显著的正相关(r=0.94002;P<0.0001);在DF-1细胞系上传的不同代数之间也呈显著正相关(r=0.96449;P=0.0080)。由此可推测ALV-B的TCID50与p27抗原呈显著正相关,可以用ELISA法测得的p27抗原的S/P值来估测病毒的TCID50值。

To study the correlation between 50% tissue-culture infective dose （TCID50） value and p27 antigen＇s S/P value of subgroup B avian leukosis virus and discuss their significance, chicken embryo fibroblast （CEF） cells were inoculated with subgroup B avian leukosis virus strain SDAU09C2 and samples were taken continuously for ten days after changing maintenance media. The correlations between TCIDs0 value and p27 antigen^s S/P value of ten days were then detected. Simultaneously, DF-1 cells were inoculated with it and passed to 20 generations. Samples taken from 1st, 5th, 10th, 15th and 20th generation were received the TCID^0 titer determination and the p27 antigen examination separately. A significant Pearson correlation was found between them in CEF cells （r=0. 85277; P〈0. 0001） and in DF-1 cells （r=0. 93000; P=0. 0220）. This study provided an important parameter for predicting TCID50 by detecting the p27 antigen＇s S/P value.