马冷冻精液氧自由基的产生和清除系统

Production and Scanvage System of Reactive Oxygen Species in Stallion Frozen Semen

马精液中存在相对稳定的氧自由基(ROS)产生和清除系统,当精液经离心去除精清后,稳定的系统遭到破坏。马精液冷冻和解冻过程不能有效的清除ROS,将导致解冻后精子各项生理功能的下降,直接影响冻精的质量,而添加外源性的抗氧化剂则可以重新使精液的ROS产生和清除达到平衡。作者综述了马精液中精子细胞、白细胞ROS的产生系统和精液GSH-PX、CAT、SOD、VA、VE等对ROS的清除系统,旨在对人们研究马精液冷冻保存时精子中ROS的损伤机理研究提供一定的帮助。

There was a relatively stable reactive oxygen species （ROS） generation and scavenging system in the stallion semen. The stable system would be destructed when seminal was removed after centrifugation. Freezing and thawing process would not be effective removal of ROS, which would lead to the thawed sperm of various physiological functions, which direct- ly affected the quality of frozen semen, by adding exogenous antioxidants could re-enable the semen of ROS generation and scavenging to achieve balance. This paper reviewed the ROS production system by stallion spermatoza and leukocyte, and ROS scavenge system by GSH-PX, CAT, SOD, VA, VE, and designed to provide some help to the people who studied stallion se- men cryopreservation of sperm by ROS injury research.