猪病毒性腹泻分子流行病学调查

Molecular Epidemiological Investigation of Swine Viral Diarrhea

为了解近年来中国猪病毒性腹泻的发生现状,于2011年4月至2012年4月利用多重RT-PCR方法对采集于13个省市的猪腹泻样品和临床健康的样品进行了检测,结果显示,腹泻猪群样品中TGEV阳性率为2.65%,PEDV阳性率为24.49%,ARV阳性率为3.20%;在肠道组织样品中,TGEV的阳性率为3.11%,PEDV为14.83%,ARV为1.67%;粪便样品中,TGEV的阳性率为2.80%,PEDV为28.42%,ARV为4.86%;母猪(所产仔猪腹泻)乳汁中,TGEV阳性率为1.08%,PEDV为31.89%,ARV为0.54%。健康猪群样品中,保育与育肥阶段猪中PEDV阳性率为2.13%,ARV阳性率为1.42%;哺乳仔猪中ARV阳性率为12.86%。由此可知,目前中国猪病毒性腹泻以PED为主要病因,PEDV和TGEV在幼龄猪群中存在隐性感染现象,且3种病毒性腹泻均可通过母乳传播病毒。

To find out the current situation of the viral diarrhea of swine in China recent years, the multiplex RT-PCR method was used to detect the samples of diarrhea pigs and clinical health pigs collected from 13 provinces and cities. The resuits were showed in the samples of diarrhea pigs, the positive rates of TGEV, PEDV and ARV were 2.65%, 24.49% and 3.20M, respectively. More specially, the positive rates of TGEV, PEDV and ARV were 3.11%, 14.83% and 1.67% respec- tively in the intestinal tissue samples; 2.80%, 28.42% and 4.86% respectively in the faeces samples and 1.08%, 31.89% and 0. 54% respectively in the milk of the sow. The detection results of the healthy pigs showed that there were still infections of PEDV and ARV, and the positive rate of PEDV was 2.13% in weaned pigs, the positive rate of ARV was 1.42% in weaned pigs and 12.86% in suckle pigs. Conclusively, PED was the major cause of swine viral diarrhea in China at present, and PEDV and TGEV might infect young pigs latently, and all the virus of these three diarrheal diseases could he spread though milk of SOW.