小桐子果壳杀虫活性部位的分离及其HPLC-MS分析

Isolation and HPLC-MS analysis insecticidal activity franctions from Japropha curcas fruit shell

为寻找小桐子果壳的杀虫活性物质,以小桐子果壳乙醇粗提物的正丁醇萃取相为实验材料,对其采用硅胶柱反复柱层析,同时对各阶段分离产物进行活性追踪,筛选出具有杀虫作用的活性部位WD1,最后采用HPLC-MS分析,初步鉴定出活性部位主体成分。结果表明,WD1活性组分在浓度为8g·L-1时,对桃蚜(Myzus persicae)和菜青虫(Pieris rapae)的24h(72h)校正死亡率均大于70%,显示出极高的毒杀活性。通过对WD1进行HPLC-MS分析,鉴定了含量较高的7个化合物,为总量的80.72%,初步鉴定为2a,3a,24-三羟基-12-20(30)-二烯-28-乌索酸、环辛肽B、牡荆素、松香烷二萜、麻疯树酚酮A、Curcusone A或Curcusone B。

To seek insecticidal activity components from Jatropha curcas fruit shell, using alcohol crude extract of bu- tanol partitioned extract in J. curcas fruit shells as an experimental material in our investigation, and then silica gel chromatography, at same time, separated products of the each stage were carried out by tracking activity, analyzed by HPLC-MS,and the main components of active part were initially identified. The results showed that the concentra- tion of colony WD1 reached 8 g·g^-1 ,Myzus persicae and Pieris rapae corrected mortality exceeded 70%in 24 h（72 h） ,which showed that toxic activity was very high. Through the analysis of WD1 by HPLC-MS, seven compounds with maximum contents were identified, with total amount up to 80.72 %. They were 2a, 3a, 23-Trihydroxyurs-12,20 （30）-dien-28-oi, Cyclgrossine B, Vitexin, Diterpene , Jatrophlone A, Curcusone A or Curcusone B.