丹霞红茶体外抗氧化与抗胃癌活性研究

Research on anti-oxidant and anti-gastric cancer activity of Danxia black tea in vitro

研究分析了丹霞系列红茶中8种儿茶素与4种茶黄素单体的含量,并用FRAP法测定丹霞红茶的总抗氧化能力,采用体外细胞培养体系MTT法检测了丹霞系列红茶对人类胃癌SGC 7901细胞生长增殖的抑制作用。结果表明,丹霞2号红茶儿茶素总量与茶黄素总量最高;总抗氧化能力由高到低顺序为丹霞1号>丹霞2号>丹霞8号>政和工夫红茶对照样;随着茶样处理浓度的增加,丹霞8号品种呈剂量依赖性地抑制了SGC 7901细胞的生长增殖,其抑制作用明显优于丹霞1号、丹霞2号红茶以及政和工夫红茶对照样,表明丹霞8号品种具有较好的抗癌作用。

The contents of eight catechins and four theaflavins in Danxia black tea were analyzed. Then, total antioxidant activity of Danxia black tea was determined using FRAP method. Finally, the effect of Danxia black tea on the growth and proliferation of human of gastric cancer SGC 7901 cells in vitro was detected by MTT assay. The result showed that Danxia black tea with the highest content of total catechines and total theaflavins was Danxia No.2, and the descending order of total antioxidant activity was Danxia No.1 〉 Danxia No.2 〉 Danxia No.8. With increasing concentrations of tea samples, Danxia NO.8 dose-dependently inhibited the proliferation of SGC 7901 cells, and the inhibition effect was more significant than that of Danxia No.1 and No.2, which suggested that Danxia NO.8 had better anticancer activity.