新疆高寒牧区产细菌素低温乳酸菌的筛选

Screening of bacteriocin-producing low-temperature lactic acid bacteria in high,cold pasture areas in Xinjiang

从新疆高寒牧区牧民自制乳制品分离得到45株乳酸菌。采用排除有机酸和过氧化氢中和试验对分离的乳酸菌进行处理,结果表明三株乳酸菌有抑菌特性。用胰蛋白酶对发酵液进行处理后,其抑菌活性大大降低,说明代谢产物是具有蛋白属性的抑菌物质,判断为细菌素。鉴定结果初步认为,这三株乳酸菌分别属于植物乳杆菌、戊糖乳杆菌和乳酸乳球菌。

Forty-five lactic acid bacteria were screened from the samples collected from the high, cold pasture areas in Xinjiang, and three of them with antimicrobial properties were screened out through the test. After exclusion of or- ganic acids and catalase, these three lactic acid bacteria still had a certain bacteriostasis performance. Meanwhile, the antibacterial activity was greatly reduced after treated with trypsin, which indicated that the metabolite was baete- riocins, the protein with antibacterial property. These three lactic acid bacteria were identified to be： Lactobacillus plantarum , Lactobacillus pentosus and Lactocoecus laetis , respectively.