摘要
目的探索Nrf2-ARE通路对肝癌HepG2细胞增生与细胞自噬的影响。为临床探索有效的肝癌综合治疗方案提供实验依据。方法培养肝癌HepG2细胞，用MTT比色法和流式细胞仪检测肝癌HepG2细胞增生抑制率及细胞周期各时相的变化。用倒置相差显微镜和荧光显微镜对肝癌细胞自噬进行定性观察。结果Nrf2抑制剂BML-111组的肝癌HepG2细胞抑制率明显高于对照组（P〈0．05），对照组的肝癌HepG2细胞抑制率明显高于Nrt2诱导剂EGb组（P〈0．05）；流式细胞仪示Nrf2抑制剂BML-111组细胞周期中G1期细胞增多，S期细胞减少，G2／M期细胞相对增多（P〈0．05）；Nrf2诱导剂EGb组细胞周期中G1期细胞减少，S期细胞增多，G2／M期细胞相对减少（P〈0．05）。倒置相差显微镜和荧光显微镜检测：Nrt2抑制剂BML-111组肝癌HepG2细胞质中有大小不等的空泡及自噬体形成。结论Nrf2-ARE通路对肝癌HepG2细胞增生及其自噬有反向抑制作用。抑制Nrf2-ARE通路后肝癌HepG2细胞多停留在细胞周期的G1期。
Objective To explore the influence of HepG2 cells＇ proliferation and autophagy by the Nrf2-ARE pathway, and provide the experimental basis for clinical exploring effective liver cancer treatment. Methods He- patic carcinoma HepG2 ceils were cultured, and its proliferation inhibition rates and the change of cell cycle＇ s in each phase were explored by the MTT assay and flow cytometry. The hepatoma cells＇ autophagy was qualitative ob- served by inverted phase contrast microscope and fluorescence microscope. Results Inhibitory rate of HepG2 cells was obviously higher in the Nrf2 inhibitor BML-111 group than control group（ P 〈 0. 05 ） , and the control group was aslo obviously higher than the Nrf2 inducer EGb group （ P 〈 0.05 ）. Flow cytometrie analysis showed that G1 phase cells in the cell cyele increased, S phase cells reduced and G2/M period cells relatively increased in the Nrf2 inhib- itor BML-111 group. But G1 phase cells reduced, S phase cells increased and G2/M period cells relative reduced in the Nrf2 inducer EGb group. Inverted phase contrast microscope and fluorescence microscope checked that ran- ging from the size of the bubble and autophagosome formed in Hepatoma HepG2 cytoplasmic of the Nrf2 inhibitor BML-111 group. Conclusions The Nrf2-ARE pathway played an reverse inhibition on HepG2 cells＇ proliferation and autophagy. After the inhibition of Nrf2-ARE pathway, HepG2 cells mostly stayed in the G1 phase of the cell cycle.
出处
《国际外科学杂志》
2013年第2期95-98,F0003,共5页
International Journal of Surgery
