聚合酶链反应检测血浆及外周血单个核细胞中HCV-RNA的意义

Detection of HCV-RNA in plasma and peripheral blood mononuclear cells in possible patients with hepatitis C

目的 为提高丙型肝炎病毒 RNA(HCV- RNA)的检出率。方法 采用套式逆转录聚合酶链反应 (RT- PCR)技术 ,检测 2 0 0例抗 - HCV阴性 ,AL T反复异常 ,临床疑似丙型肝炎患者的血浆及外周血单个核细胞 (PBMC)中 HCV- RNA。结果 5 2例患者血浆中检出 HCV - RNA,阳性率为2 6 % ,78例患者 PBMC中检出 HCV- RNA ,阳性率为 39% ,其中两者同时双阳性有 2 2例 ,阳性率为 11%。结论 同步检测丙型肝炎患者血浆及 PBMC中 HCV- RNA,在避免漏诊 ,提高临床诊断率及指导医生治疗方面是很有必要的 ,同时在研究其发病机理中有着一定意义。

Objective The aim of the study was to improve detecting rate of hepatitis C virus RNA (HCV RNA) in patients with hepatitis C. Methods Nested reverse transcriptional polymerase chain reaction(RT PCR)technique was used to detect 200 samples of plasma and peripheral blood mononuclear cell(PBMC) from possible patients with hepatitis C clinically, with anti HCV negative and with repeating alanine transferase abnormal characteristic. Results HCV RNA was positive in plasma of 52 patients with positive rate of 26%; it was also positive in PBMC of 78 patients with positive rate of 39%. There were 22 patients with positive HCV RNA both in plasma and in PBMC samples, and the positive rate was 11 percent. Conclusions Detection of HCV RNA in plasma and PBMC samples simultaneously in patients with hepatitis C may improve dianostic rate, avoid missed diagnosis, and provide doctors with a strategy for clinician to treat patients. At the same time, it was significant to study the pathogenesis of hepatitis C.